Ectopic Expression of CDF3 Genes in Tomato Enhances Biomass Production and Yield under Salinity Stress Conditions

Frontiers in Plant Science
Begoña Renau-MorataSergio G Nebauer

Abstract

Cycling Dof Factor (CDF) transcription factors (TFs) are involved in multiple processes related to plant growth and development. A member of this family, CDF3, has recently been linked in Arabidopsis to the regulation of primary metabolism and abiotic stress responses, but its role in crop production under stress is still unknown. In this study, we characterized tomato plants overexpressing the CDF3 genes from Arabidopsis and tomato and analyzed their effects on growth and yield under salinity, additionally gaining deeper insights into the molecular function of these TFs. Our results provide evidence for higher biomass production and yield in the 35S::AtCDF3 and 35S::SlCDF3 plants, likely due to a higher photosynthetic capacity resulting in increased sucrose availability. Transcriptome analysis revealed that CDF3 genes regulate a set of genes involved in redox homeostasis, photosynthesis performance and primary metabolism that lead to enhanced biomass production. Consistently, metabolomic profiling revealed that CDF3 evokes changes in the primary metabolism triggering enhanced nitrogen assimilation, and disclosed that the amount of some protective metabolites including sucrose, GABA and asparagine were higher in vegetative tiss...Continue Reading

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Citations

Feb 20, 2020·Journal of Experimental Botany·Begoña Renau-MorataJoaquín Medina
Jun 3, 2018·International Journal of Molecular Sciences·Sardar-Ali KhanHong-Ju Yin
Dec 18, 2020·Frontiers in Plant Science·José Domínguez-FigueroaJoaquín Medina
Nov 22, 2020·The Plant Journal : for Cell and Molecular Biology·Lorena Ramírez GonzalesChristian W B Bachem
Apr 20, 2021·The Plant Cell·Titouan Bonnot, Dawn H Nagel
May 22, 2021·Plant Physiology and Biochemistry : PPB·M Iqbal R KhanChirag Maheshwari
Jun 24, 2021·Trends in Plant Science·Jean-Philippe MauxionNathalie Gonzalez

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Methods Mentioned

BETA
transgenic
PCR
RNA-Seq
Illumina sequencing
salt stress
metabolomic profiling
salt

Software Mentioned

Statgraphics
AgriGO
VENNY
PlantPan2
Revigo

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