Ectopic expression of GmPAP3 alleviates oxidative damage caused by salinity and osmotic stresses.

The New Phytologist
Wing-Yen Francisca LiHon-Ming Lam

Abstract

The primary biochemical reaction of purple acid phosphatases (PAP) is to catalyze the hydrolysis of phosphate esters and anhydrides. However, the soybean GmPAP3 gene expression is induced by NaCl, osmotic, and oxidative treatments, indicating a possible role of PAP in abiotic stress responses. Confocal and electron microscopic studies demonstrated that GmPAP3 protein is mainly localized in mitochondria, a primary site for reactive oxygen species (ROS) production. When subjected to NaCl and polyethylene glycol (PEG) treatments, ectopic expression of GmPAP3 in transgenic tobacco BY-2 cells mimicked the protective effects exhibited by the antioxidant ascorbic acid: increase in the percentage of cells with active mitochondria; reduction in the percentage of dead cells; and reduced accumulation of ROS. In addition, when GmPAP3 transgenic Arabidopsis thaliana seedlings were subjected to NaCl, PEG, and paraquat (PQ) treatments, the percentage of root elongation was significantly higher than the wild type. Furthermore, PQ-induced lipid peroxidation in these transgenic seedlings was also reduced. In summary, the mitochondrial localized GmPAP3 may play a role in stress tolerance by enhancing ROS scavenging.

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Citations

Jun 12, 2012·Applied Biochemistry and Biotechnology·Asha Anand, Pramod Kumar Srivastava
May 13, 2011·Plant Physiology·William C Plaxton, Hue T Tran
Sep 1, 2015·The New Phytologist·Kwang-Moon ChoSung Han Ok
Nov 20, 2008·Journal of Integrative Plant Biology·Tsui-Hung PhangHon-Ming Lam
Jun 5, 2015·Frontiers in Plant Science·Eliécer González-MuñozRuairidh J H Sawers
Jun 5, 2013·Plant Signaling & Behavior·Adnane BargazJean-Jacques Drevon
Dec 9, 2016·International Journal of Molecular Sciences·Yuzhe SunBoon Leong Lim
Feb 15, 2019·International Journal of Molecular Sciences·Tingting JiaFuqiang Song
Apr 24, 2019·International Journal of Molecular Sciences·Chaoyan YinWenfeng Li
Mar 19, 2015·Plant Physiology·Alfredo AmbrosoneStefania Grillo
Nov 11, 2018·International Journal of Biological Macromolecules·Baskar VenkidasamySathishkumar Ramalingam
Aug 18, 2021·Plant Cell Reports·Jyoti Bhadouria, Jitender Giri

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