Ectopic Expression of Hematopoietic SHIP1 in Human Colorectal Cancer

Biomedicines
M SchaksM Jücker

Abstract

Colorectal cancer (CRC) is a heterogeneous disease that results from the accumulation of mutations in colonic mucosa cells. A subclass of CRC is characterized by microsatellite instability, which is thought to occur mainly through inactivation of the DNA mismatch repair genes MLH1 and MSH2. The inositol 5-phosphatase SHIP1 is expressed predominantly in hematopoietic cells. In this study, the expression of SHIP1 in carcinomas and its putative correlation with clinicopathologic parameters, expression of DNA repair genes and microsatellite instability was investigated. By analyzing a multi-tumor tissue microarray, expression of SHIP1 was detected in 48 out of 72 cancer entities analyzed. The expression of SHIP1 protein of 145 kDa was confirmed by Western blot analysis in 7 out of 14 carcinoma cell lines. Analysis of a large colorectal cancer tissue microarray with 1009 specimens revealed SHIP1 expression in 62% of the samples analyzed. SHIP1 expression was inversely correlated with lymph node metastasis, vascular invasion and tumor grade, and it was positively associated with left-sided tumor localization. Interestingly, a strong relationship between the expression of SHIP1 and nuclear and membranous beta-catenin and the DNA repai...Continue Reading

References

Apr 11, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ryan M O'ConnellDavid Baltimore
Apr 28, 2010·Gastroenterology·C Richard Boland, Ajay Goel
Feb 4, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Frank A Sinicrope, Daniel J Sargent
Oct 16, 2012·International Journal of Cancer. Journal International Du Cancer·Pierre TennstedtKerstin Borgmann
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Jan 21, 2015·The American Journal of Surgical Pathology·Nicole C PanarelliRhonda K Yantiss

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Methods Mentioned

BETA
Protein Assay
immunoprecipitation
phosphatase assay

Software Mentioned

R studio

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