Ectromelia virus BTB/kelch proteins, EVM150 and EVM167, interact with cullin-3-based ubiquitin ligases.

Virology
Brianne A WiltonMichele Barry

Abstract

Cellular proteins containing BTB and kelch domains have been shown to function as adapters for the recruitment of substrates to cullin-3-based ubiquitin ligases. Poxviruses are the only family of viruses known to encode multiple BTB/kelch proteins, suggesting that poxviruses may modulate the ubiquitin pathway through interaction with cullin-3. Ectromelia virus encodes four BTB/kelch proteins and one BTB-only protein. Here we demonstrate that two of the ectromelia virus-encoded BTB/kelch proteins, EVM150 and EVM167, interacted with cullin-3. Similar to cellular BTB proteins, the BTB domain of EVM150 and EVM167 was necessary and sufficient for cullin-3 interaction. During infection, EVM150 and EVM167 localized to discrete cytoplasmic regions, which co-localized with cullin-3. Furthermore, EVM150 and EVM167 co-localized and interacted with conjugated ubiquitin, as demonstrated by confocal microscopy and co-immunoprecipitation. Our findings suggest that the ectromelia virus-encoded BTB/kelch proteins, EVM150 and EVM167, interact with cullin-3 potentially functioning to recruit unidentified substrates for ubiquitination.

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Citations

Aug 8, 2008·Journal of Virology·Nick van BuurenMichele Barry
Dec 26, 2008·Journal of Virology·Alastair TealeMichele Barry
Dec 17, 2010·Journal of Virology·Logan BanadygaMichele Barry
Apr 14, 2011·Molekuliarnaia biologiia·S N Shchelkunov
Oct 15, 2014·Biomolecules·Cathal MahonElah Pick
Jan 29, 2009·FEBS Letters·Leiliang ZhangGrant McFadden
May 1, 2012·Virus Research·Natalya L VasilenkoSylvia van Drunen Littel-van den Hurk
Aug 18, 2012·Advances in Virology·Sergei N Shchelkunov
Jun 11, 2014·The Journal of General Virology·Carlos Maluquer de MotesGeoffrey L Smith
Jul 5, 2015·Archives of Virology·Justyna StruzikMarek Niemiałtowski
Aug 15, 2014·PLoS Pathogens·Nicholas van BuurenMichele Barry
Feb 14, 2014·Journal of Virology·Qian WangMichele Barry
Mar 2, 2019·The Journal of Biological Chemistry·Chen GaoStephen C Graham
Jul 3, 2021·Viruses·Carla MavianAntonio Alcamí
Aug 29, 2021·Pathogens·Sian Lant, Carlos Maluquer de Motes

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