Editing of the OsACS locus alters phosphate deficiency-induced adaptive responses in rice seedlings

Journal of Experimental Botany
Han Yong LeeGyeong Mee Yoon

Abstract

Phosphate (Pi) deficiency severely influences the growth and reproduction of plants. To cope with Pi deficiency, plants initiate morphological and biochemical adaptive responses upon sensing low Pi in the soil, and the plant hormone ethylene plays a crucial role during this process. However, how regulation of ethylene biosynthesis influences the Pi-induced adaptive responses remains unclear. Here, we determine the roles of rice 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (ACS), the rate-limiting enzymes in ethylene biosynthesis, in response to Pi deficiency. Through analysis of tissue-specific expression of OsACS in response to Pi deficiency and OsACS mutants generated by CRISPR/Cas9 [clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9] genome editing, we found that two members of the OsACS family, i.e. OsACS1 and OsACS2, are involved but differed in their importance in controlling the remodeling of root system architecture, transcriptional regulation of Pi starvation-induced genes, and cellular phosphorus homeostasis. Interestingly, in contrast to the known inhibitory role of ethylene on root elongation, both OsACS mutants, especially OsACS1, almost fail to promote lateral roo...Continue Reading

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Citations

Aug 14, 2019·International Journal of Molecular Sciences·Tsung-Meng WuChwan-Yang Hong
Nov 30, 2019·Frontiers in Plant Science·Vívian Ebeling VianaAntonio Costa de Oliveira
Jan 5, 2021·Frontiers in Plant Science·Georg JanderGyeong Mee Yoon
Feb 10, 2021·Plant Cell Reports·Xin HuangChangfu Zhu
Jun 19, 2021·Planta·Bipin K PandeyPoonam Mehra
Mar 13, 2021·Journal of Integrative Plant Biology·Dong Liu
Jul 16, 2021·Transgenic Research·Jerlie Mhay MatresInez H Slamet-Loedin
Sep 5, 2021·Physiologia Plantarum·Miguel González GuzmánVicent Arbona

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Methods Mentioned

BETA
transgenic
PCR
PCRs
targeted mutation
environmental stresses
environmental stress

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