EDTA-mediated inhibition of DNases protects circulating cell-free DNA from ex vivo degradation in blood samples

Clinical Biochemistry
Gustavo Barcelos BarraSandra Santana Soares Costa

Abstract

The extracellular DNA occurring in plasma-EDTA and serum is a biomarker of growing interest, especially in prenatal diagnosis and oncology. The objectives of the present study were to compare the DNase activity in these specimens and to investigate its ex-vivo impact over the circulating cell-free DNA yield (ccfDNA), using the circulating cell-free fetal DNA (ccffDNA) as a tool. EDTA-plasma and serum from women bearing male fetus were submitted to an endogenous DNase activity assay based on qPCR hydrolysis probe degradation, they were treated with DNAse I to investigate the action of an exogenous nuclease and also submitted to different temperature conditions to investigate the temperature-dependent degradation of the ccffDNA. In all instances, all male ccffDNA were quantified by qPCR targeting the Y chromosome-specific sequence DYS-14. Moreover, a serial dilution of EDTA was added to nonanticoagulated plasma and serum before the endogenous DNAse activity assay, to investigate the EDTA-mediated inhibition of the blood's DNase. The endogenous nuclease activity was 14.9-fold higher in serum compared to EDTA-plasma. The DNAse I treatment did not alter the ccffDNA yields in EDTA-plasma, but completely degraded it in serum. The addi...Continue Reading

References

Mar 11, 1961·Nature·R M HERRIOTTS GUPTA
Nov 17, 2006·Annals of the New York Academy of Sciences·Svetlana N TamkovichPavel P Laktionov
Jul 10, 2007·Journal of Immunological Methods·Anna CherepanovaPavel Laktionov
Oct 8, 2008·Annals of the New York Academy of Sciences·Anna V CherepanovaPavel P Laktionov
Aug 7, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Klaus JungAnja Rabien
Nov 22, 2012·Investigative Ophthalmology & Visual Science·Snehal SonawaneSandeep Jain
Jun 4, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Safia El MessaoudiAlain R Thierry
Sep 7, 2013·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Audrey ValléeMarc G Denis

❮ Previous
Next ❯

Citations

Feb 10, 2016·Molecular Oncology·Francesca RivaFrancois-Clement Bidard
May 18, 2016·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·L SorberP Pauwels
Jun 28, 2016·Molecular and Cellular Probes·Mohammad HeiatMohammad Javad Rasaee
Oct 26, 2016·Journal of Microbiological Methods·Rômulo Pessoa-E-SilvaMilena de Paiva-Cavalcanti
Oct 9, 2016·Clinical Oncology : a Journal of the Royal College of Radiologists·B O'Leary, N C Turner
Feb 1, 2017·Autoimmunity Reviews·Audrey A Margery-MuirJohn D Wetherall
Jun 9, 2016·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Charlotte FribbensNicholas C Turner
May 31, 2017·ACS Synthetic Biology·Joshua Fern, Rebecca Schulman
Jun 25, 2017·Clinical Biochemistry·David SefriouiNasrin Sarafan-Vasseur
Jul 22, 2017·Clinica Chimica Acta; International Journal of Clinical Chemistry·David SefriouiNasrin Sarafan-Vasseur
Jan 31, 2018·The Journal of Pathology·Caitlin M StewartDana Wy Tsui
Apr 5, 2018·Expert Review of Molecular Diagnostics·Frederik Banch Clausen
Mar 30, 2016·Lab on a Chip·Mokhamad Fakhrul UlumDedy Hermawan Bagus Wicaksono
Aug 25, 2018·PloS One·J BosschieterJ A Nieuwenhuijzen
Nov 22, 2018·Expert Review of Anticancer Therapy·Riziero Esposito AbateNicola Normanno
Dec 7, 2018·Metabolites·Charmion Cruickshank-QuinnNichole Reisdorph
Feb 9, 2018·Ultrasound in Obstetrics & Gynecology : the Official Journal of the International Society of Ultrasound in Obstetrics and Gynecology·Y DabiA Benachi
Mar 8, 2018·The Biochemical Journal·Hiroyuki Nakayama, Kinya Otsu
Feb 23, 2019·Clinical Chemistry·Romain MeddebAlain R Thierry
Sep 29, 2019·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·J RemonR Álvarez-Alegret
May 13, 2020·Critical Reviews in Clinical Laboratory Sciences·Vida UngererStefan Holdenrieder
Sep 13, 2019·Journal of Clinical Microbiology·Kanagavel MurugesanNiaz Banaei
Feb 14, 2017·Genes & Cancer·Junaid AnsariRodney E Shackelford
Sep 1, 2020·PloS One·Heather Wilson-RoblesGaetan Michel
Apr 14, 2017·The Journal of Infectious Diseases·Yupin TongJutta K Preiksaitis
Mar 1, 2020·Nucleic Acids Research·Kelly M HarkinsRichard E Green
Jul 17, 2020·Arteriosclerosis, Thrombosis, and Vascular Biology·Patrick HaiderPhilipp Hohensinner
Dec 13, 2017·Journal of Veterinary Internal Medicine·I J WilsonA Gal
Feb 28, 2018·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Manish KumarRosy Mondal
Apr 25, 2018·Cancer Biomarkers : Section a of Disease Markers·Takashi TakeshitaHirotaka Iwase
Sep 6, 2018·Therapeutic Advances in Medical Oncology·Raffaele PalmirottaFranco Silvestris
Dec 5, 2018·Journal of Translational Medicine·Yohann DabiAlexandra Benachi
May 26, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Bashir Mohammed AbubakarAlina Wagiran
Jun 26, 2020·Frontiers in Immunology·Maaike R ScheenstraMaarten Coorens
Nov 9, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sonya Parpart-LiLuis A Diaz
Mar 9, 2021·Expert Review of Molecular Diagnostics·Daniel AnderssonAnders Ståhlberg

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Annals of the New York Academy of Sciences
Bernhard G ZimmermannSinuhe Hahn
Fetal Diagnosis and Therapy
Ranjit AkolekarKypros H Nicolaides
Clinica Chimica Acta; International Journal of Clinical Chemistry
Safia El MessaoudiAlain R Thierry
© 2022 Meta ULC. All rights reserved