EF1α is a useful internal reference for studies of gene expression regulation in amphioxus Branchiostoma japonicum

Fish & Shellfish Immunology
Yanfeng Wang, Shicui Zhang

Abstract

Amphioxus is a well-known model organism widely used for interspecies comparative genome study, developmental homology analysis and comparative immunological investigation. However, no study has been performed so far to evaluate the internal reference for quantitative RT-PCR (qRT-PCR) studies of gene expression in this important species. In this study, two software applications (geNorm and NormFinder) were used to evaluate the expression stability of 4 housekeeping genes (ACTB, GAPDH, 18S rRNA and EF1α) in 8 different normal tissues (whole body, gut, gut-free body, hepatic caecum, gill, hind-gut, notochord and muscle) and 2 tissues (gut and gut-free body) challenged with LPS and LTA in amphioxus Branchiostoma japonicum. Our results showed that in the normal tissues, the expression of 18S rRNA was most abundant, whereas the expression levels of the other three genes were close to each other, with the expression of ACTB being most unstable. Following challenge with LPS and LTA, all the four genes exhibited varied degrees of expression changes in the different tissues and the expression stabilities of the genes were also affected by the different experimental conditions. Yet, the overall ranking results produced by the two algorit...Continue Reading

References

Mar 11, 2004·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·I M Mackay
Jun 16, 2005·Journal of Molecular Endocrinology·S A BustinM W Pfaffl
Aug 20, 2005·Advances in Physiology Education·Mark A Valasek, Joyce J Repa
May 16, 2008·Fish & Shellfish Immunology·Rosa M Jiménez-CantizanoManuel Manchado

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Citations

Jun 27, 2014·Developmental and Comparative Immunology·Na XuShicui Zhang
Mar 10, 2015·General and Comparative Endocrinology·Yu WangShicui Zhang
Oct 9, 2019·International Journal of Biological Macromolecules·Peng WangZhenhui Liu
Mar 12, 2021·Developmental and Comparative Immunology·Ying ChenGuangdong Ji
Aug 25, 2021·Developmental and Comparative Immunology·Zengyu MaShicui Zhang

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