Effect-directed analysis by high-performance thin-layer chromatography for bioactive metabolites tracking in Primula veris flower and Primula boveana leaf extracts

Journal of Chromatography. a
Ehab MahranGertrud E Morlock

Abstract

The genus Primula (Primulaceae) comprises species with high medicinal as well as ornamental values. Plants belonging to this genus are well recognized for their richness in bioactive constituents. The huge variety of secondary metabolites make their complete analysis impossible. In order to cope with this challenge, effect-directed analysis (EDA) via HPTLC coupled to structure elucidation techniques was applied on Primula species for the first time. As straightforward non-target bioanalytical technique, HPTLC-UV/Vis/FLD-EDA-ESI-HRMS hyphenates three different orthogonal dimensions, i.e. chromatography with spectrometric detection, biological/enzymatic assays and HRMS. The bioactive metabolites were determined in the middle polar extracts of two Primula species, P. veris (flower) and P. boveana (leaf). The bioactivity profiling comprised the antibacterial activity against Aliivibrio fischeri and Bacillus subtilis bacterial strains and acetyl-/butyrylcholinesterase (AChE/BChE) inhibition assays. The compounds were characterized and identified via their recorded spectral data (HRMS and 1H NMR). The results showed that linoleic and linolenic acids were the principle bioactive compounds present in the studied P. veris flower extract...Continue Reading

References

Feb 17, 2001·Phytotherapy Research : PTR·H X Xu, S F Lee
Oct 7, 2004·Phytochemistry·Vladimir D TsydendambaevAndrei G Vereshchagin
May 18, 2005·Phytochemistry·Jaromir BudzianowskiMaria Wesołowska
Sep 16, 2005·Expert Review of Neurotherapeutics·Abhilash K Desai, George T Grossberg
Oct 4, 2005·Life Sciences·Marcy J Balunas, A Douglas Kinghorn
Dec 3, 2005·International Journal of Antimicrobial Agents·T P Tim Cushnie, Andrew J Lamb
Dec 29, 2005·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Shoji KurataMasatoshi Nagai
Nov 30, 2007·Clinical Interventions in Aging·Maria Luisa OnorEugenio Aguglia
Aug 8, 2008·Journal of Enzyme Inhibition and Medicinal Chemistry·Qazi Najmus-SaqibMansoor Ahmad
Sep 4, 2009·Genes & Nutrition·Lauren Meredith WillisJames A Joseph
Mar 17, 2010·Acta Pharmaceutica : a Quarterly Journal of Croatian Pharmaceutical Society and Slovenian Pharmaceutical Society, Dealing with All Branches of Pharmacy and Allied Sciences·Natalia WszelakiAnna Karolina Kiss
Jan 15, 2011·Journal of Chromatography. a·Irena M Choma, Edyta M Grzelak
Jul 19, 2011·Mini Reviews in Medicinal Chemistry·I Erdogan OrhanE Gurkas
Sep 6, 2011·European Journal of Medicinal Chemistry·Bhuwan B Mishra, Vinod K Tiwari
Nov 18, 2011·Current Medicinal Chemistry·I Uriarte-Pueyo, M I Calvo
Jun 30, 2012·European Journal of Lipid Science and Technology : EJLST·Katrin KuhntGerhard Jahreis
Feb 16, 2013·Current Medicinal Chemistry·P RussoA Cesario
Feb 26, 2013·Archives of Pharmacal Research·Preet Anand, Baldev Singh
Nov 2, 2013·Current Neuropharmacology·Mirjana B ColovićVesna M Vasić
Jul 30, 2014·Analytical Chemistry·Gertrud E Morlock, Ines Klingelhöfer
Sep 24, 2014·Current Medicinal Chemistry·Yixi XieLicheng Ren
Oct 14, 2014·The Cochrane Database of Systematic Reviews·Man Mohan MehndirattaThierry Kuntzer
Aug 19, 2015·Biotechnology Advances·Atanas G AtanasovHermann Stuppner
Mar 10, 2016·Journal of Chromatographic Science·Dániel KrüzselyiÁgnes M Móricz
Jan 19, 2017·International Journal of Molecular Sciences·Lysanne ApelOtmar Spring
Feb 2, 2017·Phytochemical Analysis : PCA·Sarah Bräm, Evelyn Wolfram
Jun 18, 2017·Journal of Nutritional Science·A N PancheS R Chandra
Aug 15, 2017·Phytochemistry·Paola S ColomboGelsomina Fico
Apr 1, 2015·Journal of Pharmaceutical Analysis·Saikat DewanjeeTarun K Dua
Oct 23, 2018·Phytotherapy Research : PTR·Faegheh FarhadiMilad Iranshahy

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Citations

Jan 11, 2021·Journal of Chromatography. a·Vandana Gawande, Gertrud Morlock

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