Effect-directed screening of Bacillus lipopeptide extracts via hyphenated high-performance thin-layer chromatography

Journal of Chromatography. a
Maryam Jamshidi-AidjiGertrud E Morlock

Abstract

Bacillus species produce a wide array of biologically active metabolites, including nonribosomaly synthesized lipopeptides (LPs). The high-performance thin-layer chromatography (HPTLC) technique hyphenated with different bioassays and mass spectrometry was demonstrated as a valuable tool for effect-directed analysis of iturins, surfactins, fengycins and kurstakins homologues from complex mixtures of LPs. As proof of this straightforward strategy, the found surfactin and iturin A homologues were characterized and compared with reference substances. This study considered two different extraction methods for LPs produced by five Bacillus strains. The ethyl acetate extraction (Ex-1), and the acidic precipitation followed by methanol extraction (Ex-2) were investigated. Diverse enzyme inhibitions and antimicrobial potentials of LPs were analyzed, and in parallel, high-resolution mass spectra (HRMS) were online recorded from the HPTLC zones of interest. No antimicrobial effect against Gram-positive B. subtilis was evident for iturin, whereas a response was detected for surfactin. The nonpolar kurstakin compounds showed a pronounced B. subtilis antimicrobial activity in Ex-1 of almost all strains, whereas the fengycin homologues were d...Continue Reading

Citations

May 28, 2020·Frontiers in Bioengineering and Biotechnology·Beatriz Galdino RibeiroLeonie Asfora Sarubbo
May 14, 2021·Critical Reviews in Food Science and Nutrition·Chunpeng WanKannan R R Rengasamy

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