Effect evaluation of three ELISA kits in detection of paragonimiasis

Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control
Chen Mu-Xin, Chen Jia-Xu

Abstract

To evaluate the effects of 3 kinds of ELISA reagents on the detection of human paragonimiasis. A total of 45 serum samples from patients with paragonimiasis, 218 serum samples from patients with other parasitic diseases as well as 80 serum samples from healthy people were detected by GD-ELISA (IgG antigen ELISA detection reagent), ESELISA (using excretory/secretory (ES) products of Paragonimus westermani), and sAg-ELISA (using semi-purified antigen (sAg) of P. westermani), respectively. The effects of the 3 reagents were evaluated and compared. The sensitivities of GD-ELISA, ES-ELISA, and sAg-ELISA were 95.6% (95% CI:89.6%~100.0%), 93.3% (95% CI:86.0%~100.0%) and 86.7% (95% CI:76.8%~96.6%), respectively; the specificities of the above three reagents were 88.6% (95% CI:85.0%~ 92.2%), 88.9% (95% CI:85.3%~92.5%) and 99.0 % (95% CI:97.9%~100.0%), respectively, and the Youden indexes of them were 0.84, 0.82 and 0.86, respectively. sAg-ELISA is more suitable than GD-ELISA and ES-ELISA for clinical sample tests in paragonimiasis endemic areas in China.

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control
Lin AiJia-Xu Chen
Ryōikibetsu shōkōgun shirīzu
T Yoshimatsu, T Tsuda
Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control
Yan-Hong ChuJia-Xu Chen
© 2022 Meta ULC. All rights reserved