Abstract
Epidemiological studies suggest a correlation between vitamin D deficiency and colorectal cancer (CRC) incidence. The majority of sporadic tumors develop from premalignant lesions with aberrant activation of the Wnt/β-catenin signaling pathway. The adenoma cell line LT97 harbors an adenomatous polyposis coli (APC) mutation leading to constitutively active Wnt signaling. In these cells, expression of Wnt target genes leads to increased survival capacity. We hypothesized that 1,25-dihydroyvitamin D3 (1,25-D3), the active form of vitamin D3, promotes differentiation by modulating β-catenin/T-cell factor (TCF) 4-mediated gene transcription. The effect of dietary vitamin D on colonic Wnt signaling was investigated in mice fed either with 100 IU or 2500 IU vitamin D/kg diet. We examined the effect of 1,25-D3 on differentiation by measuring alkaline phosphatase activity. We analyzed mRNA expression of Wnt target genes by real time qRT-PCR. The impact of 1,25-D3 on β-catenin and TCF4 protein expression was assessed by western blot and immunohistochemistry. In LT97 cells, 1,25-D3 increased cellular differentiation and reduced nuclear β-catenin levels. Further, 1,25-D3 decreased mRNA expression of the Wnt target genes BCL-2, Cyclin D1, S...Continue Reading
References
Jun 1, 1990·Cell·E R Fearon, B Vogelstein
Sep 5, 2001·Carcinogenesis·E KallayH S Cross
Sep 3, 2002·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·M RichterB Marian
Nov 1, 2002·Cell·Marc van de WeteringHans Clevers
Apr 2, 2005·Neoplasia : an International Journal for Oncology Research·Lin ThorstensenRagnhild A Lothe
Apr 15, 2005·Nature·Tannishtha Reya, Hans Clevers
Jul 5, 2006·Cancer Research·Markus LucernaErhard Hofer
Apr 24, 2007·Carcinogenesis·Oscar AguileraAlberto Muñoz
Apr 28, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·R John MacLeodIvan Pacheco
Jun 15, 2007·Nature Reviews. Cancer·Gary J Kelloff, Caroline C Sigman
Jul 20, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Masuko Katoh, Masaru Katoh
Aug 28, 2007·Nature Reviews. Cancer·Kristin K DeebCandace S Johnson
Mar 8, 2008·Gastroenterology·David H ScovilleLinheng Li
Nov 19, 2008·The Journal of Cell Biology·Paloma Ordóñez-MoránAlberto Muñoz
Apr 28, 2009·Alimentary Pharmacology & Therapeutics·L YinH Brenner
Feb 9, 2010·The Journal of Steroid Biochemistry and Molecular Biology·María Jesús LarribaAlberto Muñoz
Apr 27, 2010·Nature Cell Biology·Louis VermeulenJan Paul Medema
Jun 10, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Fumi Takahashi-Yanaga, Michael Kahn
Feb 8, 2011·The Journal of Nutritional Biochemistry·Yanyan LiDuxin Sun
Mar 11, 2011·Nature Reviews. Cancer·Margot Zöller
Aug 23, 2011·PloS One·María Jesús LarribaHéctor G Pálmer
Mar 7, 2012·Molecular and Cellular Biology·Johan H van EsHans Clevers
Jun 1, 2012·TheScientificWorldJournal·A Manzano, P Pérez-Segura
Jul 9, 2013·Gut·Amy ReynoldsMark R Williams
Oct 22, 2013·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yi-Shiuan LiuShu-Jen Chen
Nov 10, 2013·Cancers·María Jesús LarribaAlberto Muñoz
Nov 15, 2013·Lancet·Hermann BrennerChristian Peter Pox
Feb 25, 2014·Journal of Cancer·Darina LazarovaMichael Bordonaro
Apr 3, 2014·International Journal of Cancer. Journal International Du Cancer·Irfete S FetahuEnikő Kállay
Apr 8, 2014·Nature Reviews. Cancer·David FeldmanBrian J Feldman
Aug 5, 2014·The Journal of Steroid Biochemistry and Molecular Biology·Doris HummelHenrik Csaba Horváth
Citations
Nov 30, 2016·Scientific Reports·Elisa GaucciSilvia Chichiarelli
Jun 1, 2018·International Journal of Molecular Sciences·Meenakshi UmarAouatef I Chouchane
Jul 25, 2015·Journal of Experimental & Clinical Cancer Research : CR·Bassem RefaatAthar Khojah
Aug 16, 2017·World Journal of Gastroenterology : WJG·Xue-Qi ChenJing-Nan Li
Dec 28, 2019·Nutrients·Charlotte GröschelEnikö Kallay
Aug 29, 2020·Cancers·Asunción Fernández-BarralAlberto Muñoz
Mar 28, 2020·Drug Design, Development and Therapy·Bozhi YeXueli Cai
Sep 13, 2018·Laboratory Investigation; a Journal of Technical Methods and Pathology·Luyan TangJinhua Xu
Mar 30, 2016·Cancer Prevention Research·Adel Galal El-ShemiJawwad Ahmad
Nov 25, 2020·Cancers·José Manuel González-SanchoAlberto Muñoz
Jan 19, 2021·IScience·Tara McCrayLarisa Nonn
Mar 27, 2021·The Journal of Steroid Biochemistry and Molecular Biology·Fang YuanYinxiong Li
Mar 25, 2021·Cancer Management and Research·Guangshun SunWeiwei Tang