Effect of 1,25-(OH)2D3 on Proliferation of Fibroblast-Like Synoviocytes and Expressions of Pro-Inflammatory Cytokines through Regulating MicroRNA-22 in a Rat Model of Rheumatoid Arthritis

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Ping FanYang Li

Abstract

This study aims to investigate the regulatory mechanism of 1,25-(OH)2D3 on the proliferation of fibroblast-like synoviocytes (FLS) and expressions of pro-inflammatory cytokines in rheumatoid arthritis (RA) rats via microRNA-22 (miR-22). A rat model of RA was established with a subcutaneous injection of type II collagen. After treated with different concentrations of 1,25-(OH)2D3 the proliferation of FLS was estimated by the MTT method, and the optimal concentration of 1,25-(OH)2D3 was selected for further experiments. Cell proliferation was detected by MTT. Cell cycle and apoptosis were analyzed by FCM. The IL-1β, IL-6, IL-8, and PGE2 protein expressions were determined by ELISA, and MMP-3, INOS, and Cox-2 mRNA expressions were measured by qRT-PCR. The rat model of RA was successfully established. Compared with the blank group, the 1,25-(OH)2D3 and miR-22 inhibitors groups exhibited higher proliferation inhibition and apoptosis rates, lower levels of pro-inflammatory cytokines (IL-1β, IL-6, IL-8, and PGE2), and decreased mRNA expressions of MMP-3, INOS, and Cox-2. The miR-22 mimics group had lower proliferation inhibition and apoptosis rates, elevated expressions of pro-inflammatory cytokines and MMP-3, INOS, and Cox-2 than the...Continue Reading

Citations

Aug 8, 2018·International Journal of Molecular Sciences·Steven DudicsKamal D Moudgil
May 18, 2019·Clinical Epigenetics·Shizhao LiTrygve O Tollefsbol
Jun 14, 2019·Journal of Investigative and Clinical Dentistry·Carlos Marcelo FigueredoRicardo Guimarães Fischer
Feb 6, 2020·Critical Reviews in Food Science and Nutrition·Suza Mohammad NurMohammad Imran Khan
May 2, 2020·Nutrients·Pieter-Jan MartensAnd Chantal Mathieu
May 23, 2019·Molecular Medicine Reports·Huixiao DongXiuzhen Hao
Jun 22, 2018·Frontiers in Pharmacology·Yue YangLunhao Bai
May 17, 2021·Pathology, Research and Practice·Kazem NejatiAmirAhmad Arabzadeh

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