PMID: 6412214Sep 10, 1983Paper

Effect of 2'-O-methylation on the structure of mammalian 5.8S rRNAs and the 5.8S-28S rRNA junction

Nucleic Acids Research
R N NazarT O Sitz

Abstract

The mammalian 5.8S rRNA contains a partially 2'-O-methylated uridylic acid residue at position 14 which is largely or entirely methylated in the cytoplasm (Nazar, R.N., Sitz, T.O. and Sommers, K.D. (1980) J. Mol. Biol. 142, 117-121). The effect of this methylation on the 5.8S RNA structure and 5.8-28S rRNA junction was investigated using both chemical and physical approaches. Electrophoretic studies indicated that the free 5.8S rRNA can take on at least two different conformations and that the 2'-O-methylation at U14 restricts the molecule to the more hydrodynamically open form. Structural studies using limited pancreatic or T1 ribonuclease digestion indicated that the methylated conformation was more susceptible to digestion, consistent with a more open tertiary structure. Modification-exclusion studies indicated that the first 29 nucleotides at the 5' end and residues 140 through 158 at the 3' end affect the 5.8S-28S rRNA interaction, supporting previous suggestions that the 5.8S RNA interacts with its cognate high molecular weight component through its termini. These results also suggested that the 2'-O-methylated uridylic acid residue plays a role in the 5.8S-28S rRNA interaction and thermal denaturation studies confirmed t...Continue Reading

References

Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D A Peattie
May 2, 1977·European Journal of Biochemistry·R C BrandR J Planta
Jan 28, 1970·Journal of Molecular Biology·R A Weinberg, S Penman
Sep 5, 1974·Journal of Molecular Biology·B E Maden, M Salim
Aug 5, 1974·Journal of Molecular Biology·J Speirs, M Birnstiel
Oct 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·M H VaughanJ E Darnell
Mar 10, 1971·Nature: New Biology·C P StannersH Green
Dec 16, 1969·Biochimica Et Biophysica Acta·J RetèlR J Planta
May 14, 1968·Journal of Molecular Biology·J J PeneJ E Darnell
Dec 1, 1968·Journal of Molecular Biology·R A Weinberg, S Penman
Apr 22, 1969·Biochimica Et Biophysica Acta·B G Lane, T Tamaoki
Mar 4, 1967·Nature·B G Forget, S M Weissman
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D A Peattie, W Herr
Oct 24, 1981·Nucleic Acids Research·R N Nazar, A G Wildeman
Dec 21, 1981·Nucleic Acids Research·G M VeldmanJ P Ebel
Sep 5, 1980·Journal of Molecular Biology·R N NazarK D Somers

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Citations

Jan 15, 1987·Molecular and Biochemical Parasitology·D Shippen-LentzA C Vezza
Oct 10, 2002·Biochemical and Biophysical Research Communications·Sayomi HigaTatsuo Tanaka
Sep 25, 1986·Nucleic Acids Research·H U Göringer, R Wagner
Oct 1, 1984·European Journal of Biochemistry·H U GöringerR Wagner
Jul 19, 2002·FEBS Letters·Xinyu Song, Ross N Nazar
Jul 31, 1984·Biochemistry·S D SmithT O Sitz
Oct 5, 2018·Biomolecules·Piero Lo MonacoFrédéric Catez
Oct 28, 2018·Biomolecules·S Kundhavai NatchiarBruno P Klaholz

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