Effect of 20(S)-Hydroxycholesterol on Multilineage Differentiation of Mesenchymal Stem Cells Isolated from Compact Bones in Chicken.

Genes
Roshan AdhikariWoo Kyun Kim

Abstract

Bone health and body weight gain have significant economic and welfare importance in the poultry industry. Mesenchymal stem cells (MSCs) are common progenitors of different cell lineages such as osteoblasts, adipocytes, and myocytes. Specific oxysterols have shown to be pro-osteogenic and anti-adipogenic in mouse and human MSCs. To determine the effect of 20(S)-hydroxycholesterol (20S) on osteogenic, adipogenic, and myogenic differentiation in chicken, mesenchymal stem cells isolated from compact bones of broiler chickens (cBMSCs) were subjected to various doses of 20S, and markers of lineage-specific mRNA were analyzed using real-time PCR and cell cytochemistry. Further studies were conducted to evaluate the molecular mechanisms involved in lineage-specific differentiation pathways. Like human and mouse MSCs, 20S oxysterol expressed pro-osteogenic, pro-myogenic, and anti-adipogenic differentiation potential in cBMSCs. Moreover, 20(S)-Hydroxycholesterol induced markers of osteogenic genes and myogenic regulatory factors when exposed to cBMSCs treated with their specific medium. In contrast, 20S oxysterol suppressed expression of adipogenic marker genes when exposed to cBMSCs treated with OA, an adipogenic precursor of cBMSCs. T...Continue Reading

References

May 1, 1987·Cell and Tissue Kinetics·A J FriedensteinU V Gerasimov
May 15, 1994·Genes & Development·P TontonozB M Spiegelman
Apr 1, 1996·The Journal of Clinical Investigation·R L JilkaS C Manolagas
Aug 18, 1997·Biochemical and Biophysical Research Communications·T NakamuraP Maurizio
Apr 3, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M E NuttallM Gowen
Apr 2, 1999·Science·M F PittengerD R Marshak
Jan 5, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·F ParhamiL L Demer
Mar 25, 2000·Clinical Genetics·L A Sabourin, M A Rudnicki
Jul 20, 2000·Poultry Science·C C Whitehead, R H Fleming
Feb 28, 2002·Gerontology·George K Chan, Gustavo Duque
Jun 5, 2002·Current Opinion in Lipidology·Ingemar BjörkhemUlf Diczfalusy
Oct 18, 2002·Journal of Cellular Physiology·Takahito YuasaAkira Yamaguchi
Nov 5, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Farhad ParhamiLinda L Demer
Mar 12, 2003·The Journal of Cell Biology·Cosimo De BariFrank P Luyten
Mar 11, 2004·The International Journal of Biochemistry & Cell Biology·Frank P Barry, J Mary Murphy
Apr 8, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Hoa Ton KhaFarhad Parhami
Apr 8, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Basem M AbdallahMoustapha Kassem
Apr 16, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Lin Song, Rocky S Tuan
Jun 1, 2005·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Yusuke MatsubaraYukio Akiba
Aug 16, 2005·Science·Jeong-Ho HongMichael B Yaffe
Jan 10, 2006·Cell Metabolism·Jae Myoung SuhJonathan M Graff
Apr 15, 2006·The Journal of Biological Chemistry·Eun-Joo JeonSuk-Chul Bae
Jun 24, 2006·Journal of Cellular Biochemistry·Toshihisa Komori
Aug 11, 2006·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Peter S ZammitZipora Yablonka-Reuveni
Jan 4, 2007·The Journal of Biological Chemistry·Jennifer R DwyerFarhad Parhami
Jan 24, 2007·Stem Cell Reviews·Mark L Weiss, Deryl L Troyer
Jun 15, 2007·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Tara L AghalooSotirios Tetradis
Jul 20, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Woo-Kyun KimFarhad Parhami
Sep 20, 2007·Journal of Animal Science·Z Yablonka-ReuveniG Shefer
Feb 7, 2008·PloS One·Toby G KnowlesChristine J Nicol
Apr 9, 2008·Experimental Cell Research·Eun Ji GangRita C R Perlingeiro
Apr 15, 2008·Archives of Biochemistry and Biophysics·Moustapha KassemHamid Saeed
Jul 29, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Yoshiyuki MatsubaraKoichiro Kano
Oct 16, 2008·Cellular and Molecular Life Sciences : CMLS·S MuruganandanC J Sinal
Apr 23, 2009·Stem Cells and Development·Mahesh KhatriJagdev M Sharma

❮ Previous
Next ❯

Software Mentioned

Statistics Analysis System ( SAS )

Related Concepts