Effect of 3 Preservation Methods (Freezing, Cryopreservation, and Freezing + Irradiation) on Human Menisci Ultrastructure: An Ex Vivo Comparative Study With Fresh Tissue as a Gold Standard

The American Journal of Sports Medicine
Christophe JacquetMatthieu Ollivier

Abstract

Three main meniscus preservation methods have been advocated: freezing (-80°C), freezing with gamma irradiation (-80°C + 25 kGy), and cryopreservation (-140°C). All preservation methods will result in structural and architectural properties similar to those of fresh meniscus, defined as the gold standard. Controlled laboratory study. Five human intact menisci were collected from 5 patients undergoing total knee arthroplasty. The inclusion criteria were patients <70 years old with primary unilateral (medial) femorotibial knee osteoarthritis and without surgical or traumatic history on the operated knee. Four cubes (9 mm3) were cut inside of the white, or avascular, area of each specimen's middle horn and divided into 4 groups: "fresh" control, frozen (-80°C), cryopreserved (-140°C), and frozen + irradiated (-80°C + 25 kGy). Specimens of the control group were evaluated at day 1, and specimens from the frozen, cryopreserved, and frozen + irradiated groups were evaluated after 1 month of storage. Evaluation was performed with electron microscopy according a validated protocol to analyze (1) mean diameters of the collagen fibers in longitudinal and transverse sections in 5 points per section and (2) validated architectural scores. ...Continue Reading

References

Jan 1, 1973·Journal of Clinical Pathology·M A Wallis, R L Griffin
Sep 1, 1995·The American Journal of Sports Medicine·B M FidelerT Moore
Jan 1, 1997·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·C FabbricianiM Evangelisti
May 1, 1999·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·M Krüger-FrankeB Rosemeyer
Aug 16, 2000·Clinical Orthopaedics and Related Research·H HiguchiK Takagishi
Mar 26, 2002·The American Journal of Sports Medicine·Carl Joachim WirthDieter Kohn
May 17, 2003·The American Journal of Sports Medicine·C Thomas VangsnessTillman M Moore
Apr 5, 2005·The Journal of Bone and Joint Surgery. American Volume·Peter C M VerdonkRené Verdonk
Feb 8, 2006·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Peter C M VerdonkRené Verdonk
Jan 12, 2007·International Orthopaedics·Sandro GianniniAlessandro Ruggeri
Aug 7, 2007·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Paul B LewisBrian J Cole
Dec 11, 2007·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Pablo Eduardo GelberJuan Carlos Monllau
Feb 20, 2009·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Pablo Eduardo GelberJuan Carlos Monllau
Jul 17, 2010·Osteoarthritis and Cartilage·Y KatsuragawaN Fukui
Nov 28, 2014·Methods in Molecular Biology·David E Pegg
Feb 14, 2016·Clinical Orthopaedics and Related Research·Mark D KohnNavin D Fernando
Dec 3, 2016·The American Journal of Sports Medicine·Young-Hee ChoiSi Young Song

❮ Previous
Next ❯

Citations

May 9, 2019·Orthopaedic Journal of Sports Medicine·Christophe JacquetMatthieu Ollivier
Mar 9, 2021·Tissue Engineering. Part C, Methods·M Gabriela EspinosaKyriacos A Athanasiou
Oct 17, 2020·Scientific Reports·Thomas M TiefenboeckKambiz Sarahrudi

❮ Previous
Next ❯

Methods Mentioned

BETA
transmission electron microscopy

Software Mentioned

iTEM Five
SPSS
JMP

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Related Papers

Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA
Pablo E GelberJuan Carlos Monllau
Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA
Pablo E GelberJuan Carlos Monllau
© 2022 Meta ULC. All rights reserved