PMID: 36602Apr 1, 1979

Effect of 5-bromodeoxyuridine on heterogeneous nuclear RNA in rat hepatoma cells

Nucleic Acids Research
H W Weber, R H Stellwagen

Abstract

Heterogeneous nuclear RNA HnRNA) was isolated from untreated and 5-bromodeoxyuridine (BrdUrd) treated hepatoma tissue culture (HTC) cells. analysis of this RNA by either electrophoresis on polyacrylamide-agarose gels or centrifugation in sucrose gradients demonstrated that BrdUrd caused a shift in the labeled HnRNA population toward a smaller size distribution. This effect was produced by concentrations of BrdUrd which specifically lower the level of the differentiated enzyme tyrosine aminotransferase, but do not greatly affect cell growth. Differential binding to oligo(dT) cellulose was used to fractionate HnRNA further into classes containing poly(A) (alpha), oligo(A) (beta) or neither category of A-rich sequences (gamma). BrdUrd did not alter the relative rates of uridine incorporation into the three classes. The shift in the labeled HnRNA population due to BrdUrd was observed in all three subclasses of HnRNA.

Citations

Oct 19, 1976·Biochemistry·E L KorwekS Venkatesan
Jul 5, 1975·Journal of Molecular Biology·L M Dubroff, M Nemer
Jan 1, 1970·The Journal of Cell Biology·R Bischoff, H Holtzer
Jan 1, 1973·Annual Review of Biochemistry·W J RutterP W Morris
Jun 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·R H Stellwagen, G M Tomkins
Oct 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·H S PreislerC Friend
Sep 28, 1973·Science·J E DarnellG R Molloy
Jan 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·H NakazatoD W Kopp
Sep 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·S Y Lin, A D Riggs
May 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·S Silagi, S A Bruce
Sep 28, 1972·Journal of Molecular Biology·H WeintraubH Holtzer

Related Concepts

Liver Carcinoma
Tyrosine Aminotransferase
Hepatoma, Novikoff
Centrifugation
Cell Differentiation Process
Cell Growth
Heterogeneous Nuclear RNA
Oligonucleotides
Sepharose
TAT gene

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