Effect of 50 Hz Extremely Low-Frequency Electromagnetic Fields on the DNA Methylation and DNA Methyltransferases in Mouse Spermatocyte-Derived Cell Line GC-2

BioMed Research International
Yong LiuJin-yi Liu

Abstract

Previous studies have shown that the male reproductive system is one of the most sensitive organs to electromagnetic radiation. However, the biological effects and molecular mechanism are largely unclear. Our study was designed to elucidate the epigenetic effects of 50 Hz ELF-EMF in vitro. Mouse spermatocyte-derived GC-2 cell line was exposed to 50 Hz ELF-EMF (5 min on and 10 min off) at magnetic field intensity of 1 mT, 2 mT, and 3 mT with an intermittent exposure for 72 h. We found that 50 Hz ELF-EMF exposure decreased genome-wide methylation at 1 mT, but global methylation was higher at 3 mT compared with the controls. The expression of DNMT1 and DNMT3b was decreased at 1 mT, and 50 Hz ELF-EMF can increase the expression of DNMT1 and DNMT3b of GC-2 cells at 3 mT. However, 50 Hz ELF-EMF had little influence on the expression of DNMT3a. Then, we established DNA methylation and gene expression profiling and validated some genes with aberrant DNA methylation and expression at different intensity of 50 Hz ELF-EMF. These results suggest that the alterations of genome-wide methylation and DNMTs expression may play an important role in the biological effects of 50 Hz ELF-EMF exposure.

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Citations

Oct 19, 2017·Molecular Neurobiology·Claudia ConsalesBarbara Benassi
Jun 9, 2017·Zygote : the Biology of Gametes and Early Embryos·Maxim FilatovMaria Semenova
Apr 28, 2018·Pharmacogenomics·Letícia C TavaresPaulo C J L Santos
Mar 8, 2017·Scientific Reports·Melissa ManserPrimo Schär
Mar 15, 2018·Drug Delivery·Gangliang Huang, Hualiang Huang
Jun 10, 2020·Molecular Biology Reports·Abbas KarimiMasoumeh Valipour
Dec 3, 2020·International Journal of Radiation Biology·Rohit GautamPaulraj Rajamani
Jul 22, 2021·Cell and Tissue Research·Gianfranco Giorgi, Brunella Del Re
Sep 9, 2021·Medical Oncology·Mohadeseh ShayeghanMohammad Amin Javidi

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Methods Mentioned

BETA
PCR
electrophoresis
methylation profiling
chip
GTPases

Software Mentioned

Affymetrix

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