Effect of 5,5'-diphenylhydantoin on the activities of hepatic cytosol malic enzyme and mitochondrial alpha-glycerophosphate dehydrogenase in athyreotic rats

Endocrinology
D N MannM I Surks

Abstract

Since 5,5'-diphenylhydantoin (DPH) decreases specific nuclear receptor binding of T3, the possibility that DPH might affect thyroid activity was examined by measurement of the activity of rat hepatic cytosol malic enzyme (ME) and mitochondrial alpha-glycerophosphate dehydrogenase (alpha-GPD) after DPH administration. Groups of athyreotic rats, prepared by thyroidectomy and 131I administration, were injected with vehicle (V), DPH, T3, or a combined dose of DPH and T3 for 48 h. The rats were then killed, and liver fractions were prepared for the measurement of ME and alpha-GPD activities. Compared with the V group, DPH treatment resulted in a significant increase in mean ME activity in four experiments; the mean ME activity for all studies was 2.2-fold greater than that of the V group (P less than 0.001). A significant increase in mean alpha-GPD activity was not observed in DPH-treated rats. T3 treatment resulted in a mean 31.5-fold increase in ME activity and a 10.9-fold increase in alpha-GPD activity. The magnitude of T3 induction of both enzymes was substantially attenuated by simultaneous injection of DPH, to 54-66% of the activity induced by T3 alone (P less than 0.001). Pair-feeding a T3-injected group and the DPH plus T3 g...Continue Reading

Citations

Nov 25, 2005·European Journal of Clinical Pharmacology·M Strolin BenedettiF Tonner

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