PMID: 9416791Jan 7, 1998Paper

Effect of 6(5H)-phenanthridinone, a poly (ADP-ribose)polymerase inhibitor, and ionizing radiation on the growth of cultured lymphoma cells

International Journal of Radiation Biology
D WeltinP Bischoff

Abstract

The ability of 6(5H)-phenanthridinone (Phen), a new potent poly(ADP-ribose)polymerase (PARP) inhibitor, to potentiate the effect of ionizing radiation on tumour cells was evaluated. RDM4 murine lymphoma cells were irradiated using a 60Co panoramic source and then examined for their growth, cell cycle distribution and apoptosis. Phen (100 microM) was found to inhibit more than 90% of the PARP activity in control and irradiated cells. Cell proliferation was assessed using Alamar Blue, a new fluorometric assay. Phen was found to sharply increase the radiation-induced inhibition of cell proliferation. Indeed, at 2.5 Gy the relative cell number of Phen-treated cells was 60% below control levels. At the same radiation dose, the G2M arrest was also significantly reinforced by the addition of Phen. Furthermore, this PARP inhibitor was shown to significantly increase the amount of DNA fragmentation as revealed by the DNA migration pattern in agarose gel electrophoresis. Comparable results were obtained with 3-aminobenzamide, another PARP inhibitor, but at concentrations 200-fold higher. Taken together, these results indicate the potential interest of Phen as a valuable pharmacological probe for investigating the role of PARP in cellular...Continue Reading

References

Sep 1, 1992·Cancer Metastasis Reviews·J A Hickman
Aug 1, 1989·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·L R Kelland, K S Tonkin
May 1, 1988·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·P A NelipovichS R Umansky
Mar 1, 1987·International Journal of Radiation Oncology, Biology, Physics·L Dewit
Nov 1, 1995·International Journal of Radiation Oncology, Biology, Physics·W C DeweyR E Meyn
Oct 1, 1994·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·N MirkovicL Milas
Mar 1, 1994·International Journal of Radiation Biology·L M MartinM Guichard
Jan 1, 1994·Immunology Today·T M Buttke, P A Sandstrom
May 15, 1994·International Journal of Radiation Oncology, Biology, Physics·E A BumpC N Coleman
May 26, 1993·Molecular and Cellular Biochemistry·D LautierG G Poirier
May 1, 1993·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·M R HorsmanJ Overgaard
Mar 12, 1993·International Journal of Cancer. Journal International Du Cancer·M G ChabertP Mandel
Apr 26, 1996·The Journal of Biological Chemistry·W Meikrantz, R Schlegel
Mar 1, 1996·Cancer Metastasis Reviews·R E MeynL Milas
Jan 1, 1996·International Journal of Radiation Oncology, Biology, Physics·P J Deschavanne, B Fertil

❮ Previous
Next ❯

Citations

May 22, 2009·Expert Opinion on Therapeutic Patents·Francis DumontPierre Bischoff
Mar 31, 2004·Canadian Journal of Physiology and Pharmacology·B FischerP Bischoff
Sep 7, 2020·Cancer Treatment Reviews·Laura CarrassaFrancesco Bertoni
Jun 24, 2009·Chemical Reviews·Mats Ljungman
Feb 2, 2013·Molecular Aspects of Medicine·Nicola J Curtin, Csaba Szabo
Feb 16, 2002·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Lucio TentoriGrazia Graziani
Sep 28, 2012·The Journal of Organic Chemistry·Chun LuRichard C Larock
Sep 30, 2020·Organic Letters·Xavier Abel-SnapeMark Lautens
Apr 23, 2010·Cancer Treatment Reviews·C PowellK J Harrington

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis