Effect of 710 nm visible light irradiation on neurite outgrowth in primary rat cortical neurons following ischemic insult

Biochemical and Biophysical Research Communications
Dong-Hee ChoiJongmin Lee

Abstract

We previously reported that 710 nm Light-emitting Diode (LED) has a protective effect through cellular immunity activation in the stroke animal model. However, whether LED directly protects neurons suffering from neurodegeneration was entirely unknown. Therefore, we sought to determine the effects of 710 nm visible light irradiation on neuronal protection and neuronal outgrowth in an in vitro stroke model. Primary cultured rat cortical neurons were exposed to oxygen-glucose deprivation (OGD) and reoxygenation and normal conditions. An LED array with a peak wavelength of 710 nm was placed beneath the covered culture dishes with the room light turned off and were irradiated accordingly. LED treatments (4 min at 4 J/cm(2) and 50 mW/cm(2)) were given once to four times within 8h at 2h intervals for 7 days. Mean neurite density, mean neurite diameter, and total fiber length were also measured after microtubule associated protein 2 (MAP2) immunostaining using the Axio Vision program. Synaptic marker expression and MAPK activation were confirmed by Western blotting. Images captured after MAP2 immunocytochemistry showed significant (p<0.05) enhancement of post-ischemic neurite outgrowth with LED treatment once and twice a day. MAPK act...Continue Reading

References

Nov 23, 1992·Neuroscience Letters·R P StroemerC E Hulsebosch
Jan 1, 1994·Acta Psychiatrica Scandinavica·K MishimaK Takahashi
Nov 1, 1993·Photochemistry and Photobiology·H ChaudhryI Kochevar
Jun 17, 1998·Psychiatry and Clinical Neurosciences·Y OkumotoR Nakamura
Jan 5, 2002·Journal of Clinical Laser Medicine & Surgery·H T WhelanJ Caviness
Mar 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·J T EellsH T Whelan
Apr 10, 2004·Neurological Research·Juanita J AndersShimon Rochkind
Apr 15, 2004·Neurobiology of Learning and Memory·Jess NithianantharajahMark Murphy
May 1, 2004·Journal of Clinical Laser Medicine & Surgery·Martha Simões RibeiroDenise Maria Zezell
May 25, 2004·Stroke; a Journal of Cerebral Circulation·Paul A LapchakJustin A Zivin
Feb 11, 2005·Lasers in Surgery and Medicine·Kimberly R ByrnesJuanita J Anders
Mar 11, 2008·Proceedings of the National Academy of Sciences of the United States of America·Xiaobing ChenThomas S Reese
Jan 21, 2009·Nature Reviews. Neuroscience·Wei Feng, Mingjie Zhang
Feb 6, 2009·Photomedicine and Laser Surgery·Farouk A H Al-Watban
Apr 17, 2009·BMC Complementary and Alternative Medicine·Alessandro GiulianiLaura Calzà
Sep 3, 2009·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Jieli ChenMichael Chopp
Oct 30, 2009·The British Journal of Dermatology·Y J Matthews, D L Damian
Jun 11, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Monia BarnatFatiha Nothias
Sep 7, 2011·Biochemical and Biophysical Research Communications·Hiroshi WatanabeYasunori Kanaho

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Citations

Jan 28, 2014·Journal of Photochemistry and Photobiology. B, Biology·Rendong Tang, Jiapei Dai
Jan 13, 2018·Molecular Neurobiology·Farzad SalehpourMichael R Hamblin
Aug 1, 2019·Chemical Communications : Chem Comm·Hanjun SunXiaogang Qu
Feb 6, 2015·Journal of Biomedical Optics·Noa AlonZeev Zalevsky
Feb 6, 2021·Scientific Reports·Andrea ZangariMaria Emiliana Caristo
Jul 30, 2021·Ageing Research Reviews·Fabrízio Dos Santos CardosoSérgio Gomes da Silva

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