Effect of a calcium hydroxide-based paste associated to chlorhexidine on RAW 264.7 macrophage cell line culture

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics
Raquel Assed Bezerra da SilvaAlexandra Ivo de Medeiros

Abstract

The objective of this study was to evaluate the effect of a calcium hydroxide Ca(OH)(2)-based paste (Calen) associated or not to 0.4% chlorhexidine digluconate (CHX) on RAW 264.7 macrophage cell line culture. The cell viability (MTT assay), immunostimulating properties (NO dosage), and anti-inflammatory properties (NO, TNF-alpha, and IL-1alpha dosage) were evaluated after cell exposure to the materials. Data were analyzed statistically by Kruskal-Wallis test at 5% significance level. There was low immunostimulating activity of the Calen paste associated or not to 0.4% CHX in the different materials' concentrations evaluated (P > .05). Anti-inflammatory activity with inhibition of NO and cytokine (TNF-alpha and IL1-alpha) release was observed only with Ca(OH)(2) + CHX at the highest concentration (25 microg/mL). As the Calen paste associated to 0.4% CHX did not alter cell viability or the immunostimulating and anti-inflammatory properties, the addition of CHX brought no benefits to the Ca(OH)(2)-based paste with regard to the tested parameters.

References

Oct 1, 1987·Endodontics & Dental Traumatology·U AllardT Stromberg
Jun 1, 1995·Annals of Medicine·E Moilanen, H Vapaatalo
Apr 4, 1998·European Journal of Oral Sciences·I J Blix, K Helgeland
Nov 24, 1998·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·P StashenkoR D'Souza
Apr 29, 1999·Endodontics & Dental Traumatology·R A BarkhordarM Z Hussain
Apr 20, 2001·International Endodontic Journal·W A De AlmeidaL A Silva
Jan 14, 2004·International Endodontic Journal·L A OkinoJ A P Figueiredo
Mar 19, 2005·Brazilian Dental Journal·Léa Assed Bezerra da SilvaMário Tanomaru Filho
Sep 30, 2005·Acta Odontologica Scandinavica·Till DammaschkeEdgar Schäfer
Nov 18, 2005·The Journal of Surgical Research·Charles F BellowsBernard M Jaffe
Nov 23, 2005·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Joseph A ZerellaLarz S W Spångberg
Jun 16, 2006·Journal of Oral Rehabilitation·H M TeixeiraC A De Souza Costa
Mar 23, 2007·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Luciane Dias de OliveiraMarcia Carneiro Valera

❮ Previous
Next ❯

Citations

Nov 26, 2008·Journal of Endodontics·Raquel Assed Bezerra da SilvaPaulo Tambasco de Oliveira
Dec 12, 2012·Journal of Biomedical Materials Research. Part a·Xidong CuiJack J Jiang
Nov 15, 2011·International Endodontic Journal·R K P LimaM Tanomaru-Filho
Apr 22, 2015·Journal of Endodontics·Nadia S FerreiraMarcia C Valera
May 10, 2011·Toxicon : Official Journal of the International Society on Toxinology·Karina Furlani ZoccalLúcia Helena Faccioli
Sep 10, 2009·Brazilian Dental Journal·Raquel Assed Bezerra da SilvaAlberto Consolaro

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here