PMID: 7524643Jan 1, 1994Paper

Effect of a derivatized dextran on human osteoblast growth and phenotype expression

Journal of Biomaterials Science. Polymer Edition
S BerradaM F Harmand

Abstract

Water soluble derivatized dextran named E9 with a molecular weight of 45,000 g l-1 containing 58% methyl carboxylic acid unit, 19% benzylamide unit, and 26% sulfonate with a specific anticoagulant activity of 0.29 IU mg-1 was studied for its effects on human osteoblast growth and phenotype expression for short-term treatment. At concentrations between 1 ng ml-1 and 1 microgram ml-1 E9 has no effect on DNA synthesis whereas at higher concentrations DNA synthesis is inhibited in a dose related fashion (87% for 400 micrograms ml-1). For concentrations which do not modify osteoblast growth, E9 promotes alkaline phosphatase activity, type I collagen and osteocalcin synthesis with a maximum effect for 0.1-1 microgram ml-1. It has a synergistic effect with hPTH increasing AMPc. Moreover, osteonectin synthesis was enhanced in a dose-dependent manner between 0.1 and 5 micrograms ml-1. These results seem to indicate that E9 is able to stimulate human osteoblast phenotype expression and could be useful in clinical applications.

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Citations

Dec 1, 1995·Bone·F BlanquaertJ P Caruelle
Jan 15, 2005·Journal of Biomaterials Science. Polymer Edition·Laetitia FrankJacqueline Jozefonvicz
Jul 9, 1999·Journal of Biomedical Materials Research·F BlanquaertJ P Caruelle
Oct 20, 2006·Journal of Cellular Physiology·Rebecca A JacksonSimon M Cool
Oct 1, 1995·In Vitro Cellular & Developmental Biology. Animal·S BerradaM F Harmand
Sep 1, 2007·Journal of Cellular and Molecular Medicine·D W Hutmacher, S Cool
Mar 4, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Rebecca A JacksonSimon M Cool

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