Jan 1, 1985

Effect of a hepatic activation system on the antiproliferative activity of hexamethylmelamine against human tumor cell lines

Cancer Chemotherapy and Pharmacology
K J MillerM M Ames

Abstract

Incubation of hexamethylmelamine for 1 h with human tumor cell lines in culture did not inhibit colony formation at concentrations up to the limit of drug solubility (200 micrograms/ml). When 1-h incubations were carried out in the presence of a 9,000 g rat liver supernatant preparation and an NADPH-generating system, hexamethylmelamine markedly reduced colony formation. Cyclophosphamide inhibition of colony formation was also dependent on the presence of a 9,000 g supernatant preparation and an NADPH-generating system in incubation mixtures. A 1-h incubation of N-methylolpentamethylmelamine (a DNA-alkylating metabolite formed during N-demethylation of hexamethylmelamine) with human tumor cell lines reduced colony formation in the absence of the liver-activating system. Substantial NADPH-dependent N-demethylation of hexamethylmelamine was observed with rat liver, lung, and kidney microsomal preparations. In contrast, little or no HMM metabolism was observed with tumor cells, tumor cell homogenates, or NADPH-fortified tumor cell microsomal preparations. NADPH-dependent formation of cytotoxic metabolites is a prerequisite for antiproliferative activity of hexamethylmelamine against these human tumor cell lines. In vivo activity o...Continue Reading

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Mentioned in this Paper

Metabolic Process, Cellular
Tumor Cells, Uncertain Whether Benign or Malignant
Mutamycin
Pentamethylmonomethylolmelamine
Cyclophosphamide
Lung
August Rats
Neoplasms
Hepatic
Microsomes, Liver

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