Effect of a high surface-to-volume ratio on fluorescence-based assays.

Analytical and Bioanalytical Chemistry
Radoslaw KwapiszewskiZbigniew Brzozka

Abstract

In the work discussed in this paper, the effect of a high surface-to-volume ratio of a microfluidic detection cell on fluorescence quenching was studied. It was found that modification of the geometry of a microchannel can provide a wider linear range. This is a phenomenon which should be taken into consideration when microfluidic systems with fluorescence detection are developed. The dependence of the linear range for fluorescein on the surface-to-volume ratio was determined. Both fluorescence inner-filter effects and concentration self-quenching were taken into consideration. It was found that inner-filter effects have little effect on the extent of the linear range on the microscale.

References

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Nov 13, 2003·Electrophoresis·Samuel K Sia, George M Whitesides
Nov 13, 2004·Analytical Chemistry·Madhavi KrishnanMark A Burns
Jul 28, 2006·Nature·Dirk JanasekAndreas Manz
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May 14, 2010·Analytical Chemistry·Arun AroraAndreas Manz
Jan 12, 2011·Biomedical Microdevices·Radosław KwapiszewskiZbigniew Brzózka
May 17, 2011·Clinical Biochemistry·Radoslaw KwapiszewskiZbigniew Brzozka

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Citations

May 13, 2014·Analytical and Bioanalytical Chemistry·Radoslaw KwapiszewskiZbigniew Brzozka

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Methods Mentioned

BETA
electrophoresis
chips
flow cytometry
PCR

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