PMID: 6251258Feb 1, 1980

Effect of a tsA mutation of simian virus 40 late gene expression: variations between host cell lines

Journal of Virology
J C Alwine, G Khoury

Abstract

Infection of AGMK or CV-1 cells by the early simian virus 40 mutant tsA58 at the permissive temperature (32 degrees C) followed by a shift to the nonpermissive temperature (41 degrees C) caused a substantial decrease in the levels of late viral RNA in the cytoplasm of AGMK cells but not CV-1 cells. At the translational level, this depression of late viral RNA levels was reflected by a decrease in late viral protein synthesis. Thus, in AGMK cells, an early region gene product (presumably large T-antigen) appeared to be continuously required for efficient expression of the late viral genes. In contrast, late simian virus 40 gene expression, once it is initiated in CV-1 cells, continued efficiently regardless of the tsA mutation. The difference in expression of the late simian virus 40 genes in these tsA mutant-infected monkey kidney cell lines may reflect a difference in host cell proteins which regulate viral gene expression in conjunction with early viral proteins.

Citations

Jan 1, 1982·CRC Critical Reviews in Biochemistry·B Schaffhausen

Related Concepts

Cercopithecus tantalus
Poly(A) Tail
RNA, Viral
Simian virus 40
Transcription, Genetic
Protein Biosynthesis

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