PMID: 6978151Mar 23, 1982Paper

Effect of acetate on transport of organic acid (fluorescein) in renal proximal tubules of frog

Biochimica Et Biophysica Acta
A A Nikiforov

Abstract

The effect of acetate on active fluorescein transport in intact proximal tubules of surviving frog kidney was studied. When the kidneys were incubated in a 120 mM Na+ medium, 10 mM acetate stimulated fluorescein uptake in the tubules. The stimulation was more pronounced if the kidneys had been previously preincubated for 3 h in the substrate-free solution. Lowering of the Na+ concentration in the bathing medium to 10 mM resulted in the disappearance of the acetate effect. Preincubation of the kidneys with acetate at 2-4 degrees C gave rise to stimulation of the fluorescein transport only in the 120 mM Na+ acetate-free medium. The acetate effect on the fluorescein uptake was partially prevented by ouabain. The stimulation of the uptake by acetate in the 120 mM Na+ medium correlated with an increase in the extent of reduction of pyridine nucleotides in the tubules. The pyridine nucleotides were reduced more markedly after incubation of the kidneys in the 10 mM Na+ medium, when acetate had no effect on the fluorescein transport. In both the 120 MM and the 10 mM Na+ media, the cold preincubation of the kidneys with 2.5 mM ADP or 2.5 mM ATP resulted in only slight stimulation of the fluorescein uptake. But in both media the uptake w...Continue Reading

References

Jul 31, 1978·Biochimica Et Biophysica Acta·H Löw, F L Crane
Mar 1, 1977·Proceedings of the Society for Experimental Biology and Medicine·G A GerencserS K Hong
Nov 3, 1975·Biochimica Et Biophysica Acta·V M BreslerA A Nikiforov
Sep 2, 1969·Biochimica Et Biophysica Acta·J Maxild, J V Moller
Oct 25, 1965·Biochimica Et Biophysica Acta·L J Opit, J S Charnock
May 1, 1950·Proceedings of the Society for Experimental Biology and Medicine·G E SCHREINER

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Citations

Mar 2, 1994·Biochemical Pharmacology·A A Nikiforov, I B Ostretsova
May 1, 1997·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·I B Ostretsova, A A Nikiforov
Dec 1, 1992·Biochemical Pharmacology·A A Nikiforov, I B Ostretsova
Aug 1, 1985·Biochimica Et Biophysica Acta·F L CraneH Löw

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