Effect of acidosis on chloride transport in the cortical thick ascending limb of Henle perfused in vitro

The Journal of Clinical Investigation
C S Wingo

Abstract

The present studies examined the effect of acute in vitro acidosis on chloride reabsorption in the rabbit cortical thick ascending limb of Henle (cTALH). Four protocols were used: hypercapnic acidosis; "isocapnic" peritubular acidosis (bath bicarbonate reduction to 10 mM); isocapnic luminal acidosis (luminal bicarbonate reduction to 10 mM); isocapnic peritubular acidosis in the absence of luminal potassium. Transepithelial voltage (VT) decreased during hypercapnic acidosis and increased with recovery. Chloride reabsorption (pmol X mm-1 X min-1) decreased from 50.3 +/- 8.4 to 15.7 +/- 5.6, then increased to 45.6 +/- 11.1 with recovery. Likewise, VT was decreased reversibly during isocapnic peritubular acidosis, and chloride reabsorption decreased by 60%. Chloride reabsorption was greater (28.3 +/- 3.6) when tubules were perfused at normal luminal pH than at an acidotic luminal pH (11.4 +/- 4.5; P less than 0.05). Luminal potassium removal reduced chloride transport, and acidosis had no significant additional effect. Decreased chloride reabsorption in the cTALH during acidosis could contribute to the chloruresis associated with systemic acidosis. The symmetrical nature of this effect suggests that acidosis inhibits chloride reabs...Continue Reading

References

Apr 1, 1976·The Journal of Clinical Investigation·H R Jacobson, J P Kokko
Jul 1, 1961·The Journal of Clinical Investigation·A POLAKW B SCHWARTZ

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Citations

Aug 21, 2013·Seminars in Nephrology·L Lee HammNazih L Nakhoul
Dec 1, 1988·Kidney International·P F ShanleyG C Johnson
Apr 17, 2008·Kidney International·Y Yamaji
Apr 6, 2002·American Journal of Physiology. Renal Physiology·Marc PaulaisJacques Teulon
Mar 10, 1988·The New England Journal of Medicine·D C BatlleR Gupta
Oct 19, 2020·Journal of Molecular Histology·Zinaeli López-GonzálezLaura I Escobar
Aug 1, 1989·The American Journal of Physiology·G M FeldmanA Kleinzeller

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