PMID: 11905199Jun 1, 1968Paper

Effect of acriflavin on the kinetoplast of Leishmania tarentolae. Mode of action and physiological correlates of the loss of kinetoplast DNA

The Journal of Cell Biology
L Simpson

Abstract

The loss of kinetoplast DNA in Leishmania tarentolae, which occurs in the presence of low concentrations of acriflavin, was found to be a result of selective inhibition of replication of this DNA. Nuclear DNA synthesis was relatively unaffected and cell and kinetoplast division proceeded normally for several generations. An approximately equal distribution of parental kinetoplast DNA between daughter kinetoplasts resulted in a decrease in the average amount of DNA per kinetoplast. The final disappearance of the stainable kinetoplast DNA occurred at a cell division in which all the remaining visible kinetoplast DNA was retained by one of the daughter cells. The selective inhibition of kinetoplast DNA synthesis was caused by a selective localization of acriflavin in the kinetoplast. The apparent intracellular localization of dye and the extent of uptake at a low dye concentration could be manipulated, respectively, by varying the hemin (or protoporphyrin IX) concentration in the medium and by adding red blood cell extract (or hemoglobin). Hemin and protoporphyrin IX were found to form a complex with acriflavin. During growth in acriflavin, cells exhibited an increasing impairment of colony-forming ability and rate of respiration....Continue Reading

References

Jun 1, 1965·The Journal of Cell Biology·J A Parsons
Mar 22, 1966·Biochemical and Biophysical Research Communications·T E Evans
May 1, 1961·Biophysical Journal·D FREIFELDERE P GEIDUSCHEK
Jul 1, 1961·Transactions of the Royal Society of Tropical Medicine and Hygiene·W E ORMEROD
Mar 25, 1961·Nature·P J WALKER
Jun 1, 1962·Journal of Molecular Biology·M I SIMON, H VAN VUNAKIS
Aug 20, 1963·Biochimica Et Biophysica Acta·H SAITO, K I MIURA
Feb 1, 1964·The Journal of protozoology·B M HONIGBERGD H WENRICH
Aug 1, 1964·Journal of Molecular Biology·R K TUBBSQ VANWINKLE
Jan 1, 1964·Antonie van Leeuwenhoek·C J BULDER
Jul 1, 1955·Stain Technology·W D GUDET T ODELL
Jun 1, 1962·Journal of Molecular Biology·C L SCHILDKRAUTP DOTY

❮ Previous
Next ❯

Citations

Jan 1, 1990·Teratogenesis, Carcinogenesis, and Mutagenesis·L Ebringer
Jul 1, 1969·Comparative Biochemistry and Physiology·R A BayneJ F Roberts
Feb 13, 2001·Plant Science : an International Journal of Experimental Plant Biology·M Imaizumi, J Hamada
Aug 24, 2013·Parasitology·Thiago ManchesterMaria Cristina Machado Motta
Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·K P ChangS Sassa
Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·V B Carruthers, G A Cross
Dec 1, 1970·The Journal of Cell Biology·H C Renger, D R Wolstenholme
Aug 1, 1971·The Journal of Cell Biology·H C Renger, D R Wolstenholme
May 1, 1972·The Journal of Cell Biology·P R Strauss
Feb 1, 1968·The Journal of protozoology·L Simpson
May 1, 1969·The Journal of protozoology·W Trager
Aug 1, 1969·The Journal of protozoology·G Riou, R Pautrizel
Nov 1, 1970·The Journal of protozoology·L Simpson, P Braly
Sep 20, 2011·Eukaryotic Cell·Audrey AmbitJeremy C Mottram
Dec 29, 2010·PLoS Pathogens·Arnab Roy ChowdhuryPaul T Englund
Aug 1, 1973·CRC Critical Reviews in Biochemistry·H R Mahler
Mar 28, 1971·Journal of Molecular Biology·L Simpson, A Da Silva
Apr 1, 1976·Experimental Parasitology·K P Wagner, S M Krassner
Apr 1, 1972·Experimental Parasitology·C J Bacchi, G C Hill
May 1, 1986·Chemico-biological Interactions·K ReszkaR Docampo
Aug 15, 1970·Nature·O WestergaardJ Keiding
Feb 1, 1972·Archives of Biochemistry and Biophysics·R R MeyerS J Keller
Jul 24, 1972·European Journal of Biochemistry·O Westergaard, B Lindberg
Mar 10, 1971·Nature: New Biology·P Strauss
Jan 1, 1990·Chemico-biological Interactions·R Docampo
Mar 1, 1973·Journal of Theoretical Biology·R R Meyer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.