PMID: 3765824Aug 1, 1986Paper

Effect of actinomycin D on arenavirus growth and estimation of the generation time for a virus particle

Virus Research
R LópezM T Franze-Fernández

Abstract

In an attempt to define the involvement of host transcription in arenavirus growth, a study was made of the effect of actinomycin D (AMD) on the yields of infectious Pichinde, Tacaribe and Junin viruses. The drug was added either immediately after virus adsorption or later after infection, at the stationary phase of virus growth. The time of exposure of the infected cells to the inhibitor was so chosen that the generation and release of virus into the medium took place in the presence of AMD. A double label technique was used to estimate the generation time of an arenavirus particle. This was found to be 6 h or less for all of the arenaviruses examined. The results indicated that treatment of the host cells with AMD, either immediately after virus adsorption or later after infection, does not affect the yield of infectious Pichinde, Tacaribe or Junin viruses, thus implying that continuous host transcription is not required for the replication cycle of these arenaviruses.

References

Mar 1, 1979·Virology·F SalehR W Compans
Dec 1, 1976·The Journal of General Virology·W E RawlsM J Buchmeier
Jan 1, 1975·Intervirology·S N BanerjeeW E Rawls
Jan 1, 1969·Archiv für die gesamte Virusforschung·C E Coto, M D de Vombergar
Mar 14, 1972·Biochimica Et Biophysica Acta·R P PerryJ R Greenberg
Feb 1, 1971·Proceedings of the Society for Experimental Biology and Medicine·K MifuneW Rawls

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Citations

Aug 10, 2012·Journal of Virology·Nicholas L BairdJack H Nunberg
Jul 1, 1991·Journal of Virology·S J PolyakD G Harnish

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