Effect of activation time on the nuclear remodeling and in vitro development of nuclear transfer embryos derived from bovine somatic cells

Molecular Reproduction and Development
Jong-Yeob ChoiHee-Tae Cheong

Abstract

This study was conducted to investigate the effect of recipient activation time on the chromatin structure and development of bovine nuclear transfer embryos. Serum-starved skin cells were electrofused to enucleated oocytes, activated 1-5 hr after fusion, and cultured in vitro. Some fused eggs were fixed at each time point after fusion without activation, or 3 or 7 hr after activation. Some nocodazole treated zygotes were fixed to analyze their chromosome constitutions. The proportion of eggs with a morphologically normal premature chromosome condensation (PCC) state increased 1-2 hr after fusion. Whereas eggs with elongated chromosome plate increased as activation time was prolonged to 3 hr, and 5 hr after fusion, 58.1% of eggs showed more than two scattered chromosome sets. The proportion of eggs with a single chromatin mass (40.6-56.7%) significantly increased when eggs were activated within 2.5 hr after fusion (P < 0.05). Only 23.3% of reconstituted embryos activated 5 hr after fusion formed one pronucleus-like structure (PN), whereas, 64.5-78.3% of embryos activated 1-2.5 hr after fusion formed one PN. The proportion of embryos with normal chromosome constitutions decreased as activation time was prolonged. Development rat...Continue Reading

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Citations

Jan 3, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·K I AstonK L White
Nov 17, 2010·Izvestiia Akademii nauk. Seriia biologicheskaia·G P MalenkoO I Stepanov
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May 8, 2007·Animal Reproduction Science·P C ShenW T K Cheng
Apr 1, 2015·Cellular Reprogramming·Young-Ho ChoiKatrin Hinrichs
Apr 6, 2006·Developmental Biology·Ziyi LiJohn F Engelhardt
Jul 4, 2013·Journal of Veterinary Science·In-Sun HwangHee-Tae Cheong
Oct 25, 2014·The Journal of Reproduction and Development·Satoshi AkagiSeiya Takahashi

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