PMID: 11899531Mar 20, 2002Paper

Effect of activators for activated partial thromboplastin time test on its results in the tube and automated test versions

Klinicheskaia laboratornaia diagnostika
S G VladimirovaG K Platonova

Abstract

Effects of activators used in combination with partial thromboplastin (PT), manufactured by the Kirov Institute of Hematology and Blood Transfusion, on the results of the activated partial thromboplastin time (APTT) test and its sensitivity to heparin were studied. Kaolin manufactured in Russia and by Aldrich firm and silica from Sigma were used. The method was reproduced in tubes and on Organon Teknika photooptic coagulometers: semiautomatic Coag-A-Mate XM and automated Coag-A-Mate RA-4. The concentration and optical density of the activator affected the results of APTT test, which prompts the use of kits calibrated for the PT/activator combination but not separate reagents. Combination of PT manufactured by the Kirov Institute and suspension of Russian kaolin ensured high sensitivity of the method to heparin content in the plasma and good reproducibility of the method both in tubes and on Organon Teknika coagulometers: coefficient of variation of APTT in all variants of the tube test were no higher than 10% and in the automated variant 2.6-6.2%.

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.