Effect of acute and subchronic administration of (R)-WIN55,212-2 induced neuroprotection and anti inflammatory actions in rat retina: CB1 and CB2 receptor involvement.

Neurochemistry International
Dimitris SpyridakosKyriaki Thermos

Abstract

Cannabinoids have been shown to protect the retina from ischemic/excitotoxic insults. The aim of the present study was to investigate the neuroprotective and anti-inflammatory properties of the synthetic cannabinoid (R)-WIN55,212-2 (CB1/CB2 receptor agonist) when administered acutely or subchronically in control and AMPA treated retinas. Sprague-Dawley rats were intravitreally administered (acutely) with vehicle or AMPA, in the absence or presence of (R)-WIN55,212-2 (10-7-10-4M) alone or in combination with AM251 [CB1 receptor antagonist/inverse agonist,10-4M] and AM630 (CB2 receptor antagonist,10-4M). In addition, AMPA was co-administered with the racemic (R,S)-WIN55,212 (10-4Μ). (R)-WIN55,212-2 was also administered subchronically (25,100 μg/kg,i.p.,4d) in control and AMPA treated rats. Immunohistochemical studies were performed using antibodies against the CB1R, and retinal markers for retinal neurons (brain nitric oxide synthetase, bNOS) and microglia (ionized calcium binding adaptor molecule 1, Iba1). ELISA assay was employed to assess TNFα levels in AMPA treated retinas. Intravitreal administration of (R)-WIN55,212-2 reversed the AMPA induced loss of bNOS expressing amacrine cells, an effect that was blocked by both AM251...Continue Reading

References

Jul 31, 1999·Journal of Neurochemistry·C HsiehK Mackie
Dec 14, 1999·The Journal of Comparative Neurology·S YazullaD G Deutsch
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·A StraikerG Maguire
Jan 6, 2000·Progress in Neuro-psychopharmacology & Biological Psychiatry·E S OnaiviJ R Hubbard
Apr 6, 2000·Visual Neuroscience·Q LuG Maguire
Feb 6, 2004·Journal of Neurochemistry·Keiko OhsawaShinichi Kohsaka
Feb 25, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Belén G RamírezMaría L de Ceballos
Apr 27, 2005·British Journal of Pharmacology·Juha R SavinainenJarmo T Laitinen
Aug 10, 2005·Journal of Neurochemistry·Katarzyna MareszBonnie N Dittel
Jun 27, 2007·Investigative Ophthalmology & Visual Science·Carlo NucciMauro Maccarrone
Oct 16, 2007·British Journal of Pharmacology·C BenitoJ Romero
Apr 9, 2008·Investigative Ophthalmology & Visual Science·Foteini Kiagiadaki, Kyriaki Thermos
Nov 19, 2008·Proceedings of the National Academy of Sciences of the United States of America·Lenka MikasovaOlivier J Manzoni
Oct 7, 2009·Brain : a Journal of Neurology·Javier PalazuelosIsmael Galve-Roperh
Feb 19, 2010·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Lene MartiniJennifer L Whistler
Aug 24, 2010·Nature Neuroscience·Joel E SchlosburgBenjamin F Cravatt
Aug 31, 2010·Synapse·Ester M LópezJuan J López-Costa
Feb 26, 2011·Molecular Pharmacology·Ana María Martín-MorenoMaría L de Ceballos
Jun 7, 2011·Biochimica Et Biophysica Acta·Natasha L GrimseyMichelle Glass
Nov 15, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Young C ChungByung K Jin
Nov 21, 2013·Investigative Ophthalmology & Visual Science·Bruno CécyreChristian Casanova
Feb 6, 2014·Experimental Eye Research·Pedro LaxNicolás Cuenca

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.