PMID: 2498462Jun 1, 1989Paper

Effect of acute ethanol on release of endogenous adenosine from rat cerebellar synaptosomes

Journal of Neurochemistry
M Clark, M S Dar

Abstract

The effects of pharmacologically relevant concentrations of ethanol on the release of endogenous adenosine from rat cerebellar synaptosomes were investigated. Release was conducted for 5, 10, 30, or 60 s after which time the incubation medium (containing the released adenosine) was rapidly separated from the synaptosomal membranes by vacuum filtration. The adenosine content of the filtrate was measured by HPLC-fluorescence detection. Both basal and KCl-stimulated adenosine release consisted of an initial rapid phase, for the first 10 s, that was followed by a relatively slower phase. Basal endogenous adenosine release was estimated as 199 +/- 14 pmol/mg protein/5 s. Potassium (chloride) increased adenosine release from the basal level to 433 +/- 83 pmol/mg protein/5 s. Ethanol caused a dose-dependent increase of adenosine release. The interaction between dilazep and ethanol indicates that ethanol-stimulated release does not involve the dilazep-sensitive transport system. The results support previous findings that indicate that cerebellar adenosine is involved in the mediation of ethanol-induced motor disturbances in the rat.

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Citations

Sep 4, 1992·European Journal of Pharmacology·D Martin, H S Swartzwelder
Feb 17, 1995·Neuroscience Letters·J M Brundege, T V Dunwiddie
Jan 23, 2015·Addiction Biology·Rose ChesworthAndrew J Lawrence
Jun 21, 2005·Pharmacology & Therapeutics·George Robert SigginsZhiguo Nie
Apr 2, 2015·Alcoholism, Clinical and Experimental Research·Yedy IsraelMario Herrera-Marschitz
Apr 18, 2015·Reproductive Toxicology·Aline Haab LutteRosane Souza Da Silva
Jan 30, 2018·Frontiers in Pharmacology·Inmaculada Ballesteros-YáñezStefania Gessi
Dec 1, 1992·Alcoholism, Clinical and Experimental Research·M S Dar, M Clark
Jun 1, 1995·Alcoholism, Clinical and Experimental Research·J Anwer, M S Dar
Jul 19, 2011·Alcoholism, Clinical and Experimental Research·Tracy R Butler, Mark A Prendergast
Aug 3, 2013·Alcoholism, Clinical and Experimental Research·M Saeed Dar
Jan 13, 2011·Alcoholism, Clinical and Experimental Research·Liana AsatryanDoo-Sup Choi

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