Effect of adenine nucleotides on cyclooxygenase and lipoxygenase enzyme products of arachidonic acid in human platelets

Biochemical Pharmacology
Y P Dragan, E F Ellis

Abstract

Nucleotides are known to enhance cyclooxygenase product formation in several tissues and, in addition, are believed to function as cofactors for mammalian 5-lipoxygenases. Since nucleotides are released by stimulated platelets and by damaged tissue, we examined the hypothesis that nucleotides can affect the metabolism of arachidonic acid (AA) in washed human platelets. The various nucleotides were given 15 sec prior to the addition of 3 microM arachidonic acid and 1 muCi [3H]AA. We found that the phosphorylated adenine derivatives (ATP, ADP, and AMP) increased the formation of 12-hydroxyeicosatetraenoic acid (12-HETE) by 2-fold without altering the formation of cyclooxygenase products. Adenosine was without effect on 12-HETE formation. ATP also stimulated 12-HETE formation in lysed platelets. This suggests that the 12-lipoxygenase enzyme of platelets can be regulated by adenine nucleotides. We next determined the portion of the nucleotide molecule responsible for the enhanced 12-lipoxygenase activity of platelets. Alteration of the nucleotide base led to a decrease in stimulation, with GTP less active than ATP, and UTP even less active than GTP. Studies with adenine nucleotides showed that the length of the phosphate chain was ...Continue Reading

References

Apr 1, 1976·The Journal of Pharmacy and Pharmacology·Y Kamikawa, Y Shimo
Oct 1, 1976·Canadian Journal of Physiology and Pharmacology·I H ChaudryA E Baue
Feb 14, 1979·Biochemical and Biophysical Research Communications·E S KangA H Kang
Jul 1, 1979·Archives of Biochemistry and Biophysics·T M ChiangA H Kang
Jan 1, 1979·Annual Review of Medicine·H Holmsen, H J Weiss
Oct 1, 1978·The Journal of Experimental Medicine·E Remold-O'Donnell
Oct 21, 1976·Journal of Theoretical Biology·G Burnstock
Jan 1, 1985·Annual Review of Physiology·J D Pearson, J L Gordon
Jan 15, 1986·The Biochemical Journal·J L Gordon
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·E J ForsbergH B Pollard
Jan 29, 1986·Biochemical and Biophysical Research Communications·M J Otero, L Carrasco
Dec 6, 1974·Nature·E G Trams
Feb 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M HambergB Samuelsson
Jan 1, 1970·Biochimica Et Biophysica Acta·I H Chaudry, M K Gould
Oct 1, 1984·British Journal of Pharmacology·P G Hellewell, J D Pearson
Jul 16, 1982·Biochimica Et Biophysica Acta·C H Emes, N Crawford
Jun 19, 1980·Biochimica Et Biophysica Acta·S R Ayad, R J Hughes
Apr 15, 1983·Biochemical and Biophysical Research Communications·J M Boeynaems, N Galand
Dec 7, 1980·Journal of Theoretical Biology·E G Trams
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P DickerE Rozengurt
Sep 11, 1981·European Journal of Pharmacology·M SchwartzmanA Raz
Mar 20, 1980·Biochimica Et Biophysica Acta·I H Chaudry, A E Baue
Jan 1, 1962·Acta Physiologica Scandinavica. Supplementum·K GRETTE
Apr 1, 1947·The American Journal of Physiology·H M KALCKAR, O H LOWRY

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.