Effect of adenosine monophosphate, adenosine diphosphate, and reduced nicotinamide adenine dinucleotide on adenosine triphosphate-dependent carbon dioxide fixation in the autotroph Thiobacillus neapolitanus.

Journal of Bacteriology
J V Mayeux, E J Johnson

Abstract

The observation that adenosine triphosphate (ATP)-dependent CO(2) fixation in extracts of chemosynthetic and photosynthetic autotrophs may be regulated in part by adenosine monophosphate (AMP) was extended to the strict autotroph Thiobacillus neapolitanus (X). In addition, this report presents data which include adenosine diphosphate (ADP) in the regulatory role. When the primary CO(2) acceptor, ribose-5-phosphate, was replaced by ribulose-1,5-diphosphate, no inhibition of CO(2) fixation occurred unless the Mg(++) concentration was limiting. A molar ratio of 5:1 AMP or ADP to ATP reduced the specific activity (micromoles of CO(2) fixed per milligram of protein per minute) of the extracts from 0.22 to 0.12 and 0.11, respectively. The reported stimulation of the carboxylative phase of ATP-dependent CO(2) fixation by reduced nicotinamide adenine dinucleotide (NADH(2)) was investigated. Adding NADH(2) to the extracts did not stimulate CO(2) fixation, even at carbonate levels from 0.05 to 30 mumoles, except in the absence of ribose-5-phosphate. Slight increases in CO(2) fixation were noted when the assay system was incubated in air instead of the usual helium atmosphere.

References

Aug 16, 1965·Biochemical and Biophysical Research Communications·M I Aleem, E Huang
Sep 8, 1966·Biochemical and Biophysical Research Communications·N L Gale, J V Beck
Apr 1, 1963·Journal of Molecular Biology·J MONODF JACOB
May 1, 1965·Journal of Molecular Biology·J MONODJ P CHANGEUX
Aug 1, 1959·Journal of Bacteriology·M SANTERU SANTER

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Citations

Jan 1, 1972·Zeitschrift für allgemeine Mikrobiologie·O H Tuovinen, D P Kelly
Apr 1, 1968·Journal of Bacteriology·M K JohnsonB S Bruff
Feb 1, 1971·Archives of Biochemistry and Biophysics·A S Cornish, E J Johnson

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