PMID: 8967815May 1, 1996Paper

Effect of adenosine on glycolysis of cultured cardiac myocytes of newborn rats

Archivos del Instituto de Cardiología de México
A Díaz, J Suárez

Abstract

Adenosine is a hormonal modulator of metabolic processes. In some tissues the effect of adenosine on glycolysis is well established. Nevertheless, there are controversial data in heart. We believe that these discrepancies in the literature are due to haemodynamic factors which are not unified in those reports. We studied the direct effect of adenosine in cardiac myocytes in culture, isolated from neonatal rat heart, which are not influenced by haemodynamics forces as whole heart. Myocytes were isolated using conventional methods. Glycolysis was measured following the formation of tritium water from D-[3-3H]glucose. The results obtained showed that the nonmetabolizable adenosine analogue, N6-(L-2-phenylisopropyl)adenosine, (10 microM) stimulates basal glycolysis (49.86%). This effect was observed in the absence or presence of (5 mM) glucose. In separated group of experiments we examined different concentrations of N6-(L-2-phenylisopropyl)adenosine, (1, 10 and 100 microM, glucose 5 mM). Concentrations of 1 y 10 microM of the adenosine analogue stimulated glycolysis in a concentration-dependent fashion, however 100 microM of N6-(L-2-phenylisopropyl)adenosine inhibited glycolysis by 83% possibly through a toxic effect on the myocar...Continue Reading

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.