Effect of adenosine triphosphate-sensitive potassium activation on peripheral and central pain sensitization

The Journal of Surgical Research
Shiren ShenJunjie Chen

Abstract

Alterations in adenosine triphosphate-sensitive potassium (KATP) activity and expression under changing physiological conditions are important adaptive and protective mechanisms. KATP subunit expression is also altered in neuropathic pain; whether these changes are adaptive or deleterious is unclear. We therefore established a skin/muscle incision and retraction (SMIR) rat model of postoperative pain and examined the relationship between pain sensitization and changes in KATP subunit expression. Rats were randomly divided into untreated, sham-operation, SMIR, and SMIR + Pinacidil (sulfonylurea receptor [SUR]2-activator) groups. In the SMIR group, skin and muscle were retracted for 1 h after incision. In the SMIR + Pinacidil group, Pinacidil was injected intraperitoneally 0.5 h before SMIR or into the spinal myelin sheath 7 d after SMIR. Mechanical withdrawal threshold was used as an index of pain sensitivity. Expression levels and localization of the KATP subunits Kir6.2, Kir6.1, SUR1, and SUR2 were measured by Western blotting and immunofluorescence. A rat postoperative pain model was successfully established, in which SMIR induced mechanical hypersensitivity (allodynia). Notably, significantly increased Kir6.1, Kir6.2, SUR1, ...Continue Reading

References

Oct 17, 1980·European Journal of Pharmacology·J L Hylden, G L Wilcox
May 6, 2008·Neuroscience Letters·Yong-Jing Gao, Ru-Rong Ji
Oct 30, 2008·Molecular Pain·Andre LaferrièreTerence J Coderre
Sep 30, 2009·IUBMB Life·Alejandro AkrouhMonica Sala-Rabanal
Jan 28, 2010·Molecular Pain·Vasiliki ZogaConstantine Sarantopoulos
Oct 10, 2013·European Journal of Pain : EJP·D CastelS Meilin
Jan 8, 2014·Current Neuropharmacology·Xiaona Du, Nikita Gamper

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