Effect of aflatoxin B1 , benzo[a]pyrene, and methapyrilene on transcriptomic and epigenetic alterations in human liver HepaRG cells

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
Volodymyr TryndyakIgor P Pogribny

Abstract

The increasing number of man-made chemicals in the environment that may pose a carcinogenic risk highlights the need for developing reliable time- and cost-effective approaches for carcinogen detection and identification. To address this issue, we investigated the utility of high-throughput microarray gene expression and next-generation genome-wide DNA methylation sequencing for the in vitro identification of genotoxic and non-genotoxic carcinogens. Terminally differentiated and metabolically competent human liver HepaRG cells were treated at minimally cytotoxic concentrations of (i) the genotoxic human liver carcinogen aflatoxin B1 (AFB1) and its structural non-carcinogenic analog aflatoxin B2 (AFB2); (ii) the genotoxic human lung carcinogen benzo[a]pyrene (B[a]P) and its non-carcinogenic isomer benzo[e]pyrene (B[e]P); and (iii) the non-genotoxic liver carcinogen methapyrilene for 72 h and transcriptomic and DNA methylation profiles were examined. Treatment of HepaRG cells with the liver carcinogens AFB1 and methapyrilene generated distinct gene-expression profiles, whereas B[a]P had only a slight effect on gene expression. In contrast to transcriptomic alterations, treatment of HepaRG cells with the carcinogenic and non-carci...Continue Reading

Citations

Dec 18, 2019·Journal of Applied Toxicology : JAT·Katrin KreuzerLinda Böhmert
Jun 23, 2020·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Kaat LeroyMathieu Vinken
Sep 15, 2020·Critical Reviews in Toxicology·Akin Cayir
Aug 28, 2020·Archives of Toxicology·Volodymyr P TryndyakIgor P Pogribny
Jan 16, 2021·International Journal of Environmental Research and Public Health·Oluwakemi A RotimiJaclyn M Goodrich
Apr 3, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Katrin KreuzerAlbert Braeuning
Nov 10, 2020·Toxicology in Vitro : an International Journal Published in Association with BIBRA·D DesaulniersC Parfett
Sep 2, 2021·Critical Reviews in Toxicology·Akin Cayir
Sep 7, 2021·Analytical Methods : Advancing Methods and Applications·Nicolás Mateo González-LópezZuly Jenny Rivera-Monroy

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