Effect of aflatoxin B1 treatment in vivo on the in vitro activity of hepatic and extrahepatic glutathione S-transferase

Toxicology Letters
M C CarrilloJ A Monti

Abstract

The effect of aflatoxin B1 (AFB1) on the glutathione S-transferase activity (GST) and on non-protein thiol levels of different tissues was studied in adult male Wistar rats. Animals received a single dose of the toxin (100 or 500 micrograms/kg body wt., p.o.), and were studied 6 or 24 h after administration. GST was determined in liver, renal cortex, duodenum, jejunum-ileum and distal ileum, using 3 substrates: 1-chloro-2,4-dinitrobenzene (CDNB), trans-4-phenyl-3-buten-2-one (PBO) and 1,2-epoxyethylbenzene (STOX). The non-protein thiol content of all tissues tested increased with the lowest dose at 6 h, returning to normal values at 24 h, while the higher dose produced a significant decrease in reduced thiol levels at 6 h, returning to normal values at 24 h. AFB1 administration induced, independently of dose and tissue, total GST (CDNB) and epoxide-transferase activity (STOX) while A--C-type transferases (PBO) were inhibited. Almost all activities returned to normal values at 24 h. In cases of enzyme induction there was in general an increase in Vmax and a decrease in apparent Km. The opposite was seen in cases of inhibition. In conclusion, the results provide evidence that extrahepatic GST could be important in the overall pro...Continue Reading

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Citations

Apr 1, 1994·European Journal of Drug Metabolism and Pharmacokinetics·S RaisuddinP K Ray
Oct 19, 2006·Plant Foods for Human Nutrition·Anaberta Cardador-MartínezGuadalupe Loarca-Piña
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Feb 7, 2008·Liver International : Official Journal of the International Association for the Study of the Liver·Mohanasundaram UmaraniPanchanadham Sachdanandam
Jul 7, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Guylaine M MeissonnierPierre Galtier
May 28, 2020·Animals : an Open Access Journal From MDPI·Enrico GugliandoloRosalia Crupi
Dec 22, 1999·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·B Premalatha, P Sachdanandam

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