Effect of age and food restriction on alkaline protease activity in rat liver

The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
T Shibatani, W Ward

Abstract

The effect of age and food restriction on the hepatic alkaline protease activity of 100,000 x g supernatant has been investigated using 7-, 16-, and 26-month-old Fischer 344 rats. The proteasome, a major component of alkaline protease activity, is activated by sodium dodecyl sulfate (SDS) and this property was exploited to gain insight into the effects of age and food restriction on proteasome activity. Three alkaline protease activities, chymotrypsin-like (ChT-L), trypsin-like (T-L), and peptidylglutamyl peptide hydrolyzing (PGPH) activities were measured. These activities are also commonly used as measurement of proteasomal activities. Basal ChT-L and PGPH activities were not markedly altered by either age or food restriction. The level of T-L activity did not change with age, but was decreased by food restriction. SDS-activated ChT-L activity increased 15% between 7 and 26 months of age and this increase was blocked by food restriction. SDS-activated PGPH activity decreased 40% and the decrease was ameliorated by food restriction. In conclusion, we have shown that the alteration of alkaline protease activities by age and food restriction is not uniform and that the changes observed are likely due to alterations of proteasoma...Continue Reading

Citations

Jun 11, 1998·Mechanisms of Ageing and Development·M M ScrofanoA Taylor
Jul 15, 1998·Mechanisms of Ageing and Development·T Hayashi, S Goto
Dec 23, 1998·Mechanisms of Ageing and Development·M M ScrofanoA Taylor
Apr 18, 2000·Experimental Gerontology·A M Cuervo, J F Dice
Sep 9, 2000·Experimental Gerontology·A J Lambert, B J Merry
Nov 10, 2009·Mechanisms of Ageing and Development·Kalavathi DasuriJeffrey N Keller
Oct 30, 2007·Ageing Research Reviews·Suresh I S Rattan
Apr 8, 2015·Mechanisms of Ageing and Development·Valerie VanhoorenBertrand Friguet
Nov 7, 2001·Clinical and Experimental Dermatology·A Stolzing, T Grune
Jul 27, 2000·Annals of the New York Academy of Sciences·B FriguetI Petropoulos
Nov 1, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Sharon JudgeChristiaan Leeuwenburgh

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