Effect of age on basal muscle protein synthesis and mTORC1 signaling in a large cohort of young and older men and women

Experimental Gerontology
Melissa M MarkofskiElena Volpi

Abstract

The rate of muscle loss with aging is higher in men than women. However, women have smaller muscles throughout the adult life. Protein content is a major determinant of skeletal muscle size. This study was designed to determine if age and sex differentially impact basal muscle protein synthesis and mammalian/mechanistic target of rapamycin complex 1 (mTORC1) signaling. We performed a secondary data analysis on a cohort of 215 healthy, non-obese (BMI<30kg·m(-2)) young (18-40y; 74 men, 52 women) and older (60-87y; 57 men, 32 women) adults. The database contained information on physical characteristics, basal muscle protein fractional synthetic rate (FSR; n=215; stable isotope methodology) and mTORC1 signaling (n=125, Western blotting). FSR and mTORC1 signaling were measured at rest and after an overnight fast. mTORC1 and S6K1 phosphorylation were higher (p<0.05) in older subjects with no sex differences. However, there were no age or sex differences or interaction for muscle FSR (p>0.05). Body mass index, fat free mass, or body fat was not a significant covariate and did not influence the results. We conclude that age and sex do not influence basal muscle protein synthesis. However, basal mTORC1 hyperphosphorylation in the elderl...Continue Reading

References

May 31, 2001·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·W W CampbellW J Evans
Jun 30, 2001·The Journal of Endocrinology·S BhasinT W Storer
Sep 18, 2001·JAMA : the Journal of the American Medical Association·E VolpiR R Wolfe
Oct 14, 2003·Journal of Applied Physiology·Fulvio LauretaniLuigi Ferrucci
Mar 23, 2004·Journal of Applied Physiology·Jascha D ParkingtonRoger A Fielding
Aug 21, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Christelle GuilletYves Boirie
Dec 15, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Daniel CuthbertsonMichael J Rennie
Jul 30, 2005·The Journal of Physiology·Paul L KimStuart M Phillips
Sep 1, 2005·American Journal of Physiology. Endocrinology and Metabolism·Benjamin F MillerMichael Kjaer
Nov 25, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Katsuhiko FunaiRoger A Fielding
May 18, 2006·American Journal of Physiology. Endocrinology and Metabolism·Satoshi FujitaElena Volpi
Aug 10, 2006·American Journal of Physiology. Endocrinology and Metabolism·Satoshi FujitaElena Volpi
Mar 8, 2008·Journal of Applied Physiology·Micah J DrummondBlake B Rasmussen
Aug 30, 2008·The Australian Journal of Physiotherapy·Paul SmithMichael Dorevitch
Oct 2, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Gregory C HendersonK Sreekumaran Nair
May 28, 2009·The Journal of Clinical Endocrinology and Metabolism·Christelle GuilletYves Boirie
Oct 8, 2009·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Angela Chalé-RushRoger A Fielding
Oct 30, 2009·The American Journal of Clinical Nutrition·Matthew J DelmonicoUNKNOWN Health, Aging, and Body
Feb 13, 2010·Journal of Applied Physiology·Christopher S FryBlake B Rasmussen
Jun 4, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Erin L GlynnBlake B Rasmussen
Mar 26, 2011·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Stéphanie ChevalierJosé A Morais
May 25, 2011·Medicine and Science in Sports and Exercise·Dillon K WalkerBlake B Rasmussen
Jul 30, 2011·Skeletal Muscle·Christopher S FryBlake B Rasmussen
Jan 17, 2012·Medicine and Science in Sports and Exercise·Gordon I SmithBettina Mittendorfer
Mar 3, 2012·Journal of Applied Physiology·Daniel W D WestStuart M Phillips
May 10, 2012·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Wilson C FokViviana I Pérez
May 25, 2012·Biology of Sex Differences·Gordon I SmithBettina Mittendorfer
Aug 15, 2012·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Jared M DickinsonBlake B Rasmussen
Sep 11, 2012·Metabolism: Clinical and Experimental·Jared M DickinsonBlake B Rasmussen
Sep 25, 2012·Mechanisms of Ageing and Development·Mengyao LiNicolas Musi
Oct 24, 2012·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Christopher S FryBlake B Rasmussen

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Citations

Jun 5, 2016·Experimental Gerontology·Derek M HuffmanNathan K LeBrasseur
Jul 20, 2016·Annual Review of Nutrition·Brandon J F GhellerAnna E Thalacker-Mercer
Nov 4, 2016·Amino Acids·Antonio Herbert LanchaFrancois Blachier
Jan 20, 2017·Pflügers Archiv : European journal of physiology·Kunihiro SakumaAkihiko Yamaguchi
Nov 21, 2015·Inflammatory Bowel Diseases·Vandre C FigueiredoDavid Cameron-Smith
Nov 15, 2018·Physiological Reviews·Lars LarssonMarco Sandri
Jun 16, 2016·F1000Research·James A Timmons, Iain J Gallagher
Aug 30, 2017·The Proceedings of the Nutrition Society·Mette Hansen
Apr 17, 2019·Journal of Applied Physiology·Anthony J SannicandroKatarzyna Goljanek-Whysall
Dec 25, 2015·Journal of Applied Physiology·Gordon I Smith, Bettina Mittendorfer
Nov 3, 2016·American Journal of Physiology. Endocrinology and Metabolism·Brandon J ShadLeigh Breen
Oct 5, 2019·Journal of Bone and Mineral Metabolism·Mitsutaka YakabeSumito Ogawa
Jul 10, 2019·The Proceedings of the Nutrition Society·Oliver C WitardStuart R Gray
Nov 14, 2019·Proteomics·Kuan Ting TanShih-Yin Tsai
Jul 2, 2020·American Journal of Physiology. Cell Physiology·Evelyn ZacharewiczSéverine Lamon
Mar 25, 2020·Medicine and Science in Sports and Exercise·Jaakko HentiläMarko T Korhonen
Nov 2, 2017·Frontiers in Physiology·Mee-Sup Yoon
Aug 15, 2020·Frontiers in Physiology·Yori EndoIndranil Sinha
Dec 7, 2016·Menopause : the Journal of the North American Menopause Society·Thaís R Silva, Poli M Spritzer
Feb 18, 2016·Biogerontology·Oliver C WitardStuart M Phillips
Jul 25, 2018·Nutrients·Mary Ni LochlainnClaire J Steves
Nov 19, 2015·American Journal of Physiology. Endocrinology and Metabolism·Benjamin T WallLuc J C van Loon
Feb 15, 2017·Journal of the International Society of Sports Nutrition·T N ZiegenfussA A Ferrando
Sep 29, 2020·Frontiers in Molecular Neuroscience·Perrine CastetsMarkus A Rüegg
Aug 14, 2015·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·David L WilliamsonJacob M Haus
Nov 2, 2019·Journal of Applied Physiology·Douglas W Van PeltTimothy A Butterfield
Sep 11, 2020·Nature Communications·Daniel J HamMarkus A Rüegg
Feb 20, 2019·International Journal of Molecular Sciences·Francesca ChiariniGiovanna Lattanzi
Oct 17, 2020·Current Opinion in Clinical Nutrition and Metabolic Care·Chiara RuoccoEnzo Nisoli
Nov 18, 2020·Menopause : the Journal of the North American Menopause Society·Thaís R SilvaPoli Mara Spritzer

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