Effect of ageing and caloric restriction on specific markers of protein oxidative damage and membrane peroxidizability in rat liver mitochondria

Mechanisms of Ageing and Development
A J LambertB J Merry

Abstract

To gain insight into the anti-ageing mechanisms of caloric restriction (CR), liver mitochondria were isolated from male Brown-Norway rats of different ages (fully fed control and CR) and various specific markers of non-enzymatic protein modification (by oxidative, glyco- and lipoxidative-reactions) were measured by GC/MS and Western blotting. A membrane peroxidizability index (PI) was calculated from the fatty acid profiles. Between 6 and 18 months of age, there were significant decreases in the concentration of all markers of damage in mitochondria from both the fully fed and CR groups. In contrast, between the ages of 18 and 28 months, there were significant increases in the concentrations of all markers of damage. In mitochondria from both fully fed and CR groups, there were significant increases in N-epsilon (Nepsilon)-(carboxymethyl)lysine (CML) and N-epsilon-(malondialdehyde)lysine (MDAL) between 6 and 28 months of age. In general, damage tended to be lower in mitochondria from CR animals, but the effects were not significant, except for the concentration of N-epsilon-(carboxymethyl)lysine at 28 months of age. PI increased steadily and significantly with age in fully fed animals, whilst CR induced a significant decrease i...Continue Reading

References

Jan 1, 1975·Advances in Experimental Medicine and Biology·P D Wilson, L M Franks
Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·L D YoungmanB N Ames
Jan 1, 1992·Free Radical Biology & Medicine·M H ChungB P Yu
Feb 1, 1991·Archives of Biochemistry and Biophysics·G Paradies, F M Ruggiero
Dec 1, 1989·Archives of Biochemistry and Biophysics·P E Starke-Reed, C N Oliver
May 1, 1987·Lipids·J P CosgroveW A Pryor
Jun 30, 1987·Biochemical and Biophysical Research Communications·S Laganiere, B P Yu
Dec 1, 1993·Mutation Research·V Haley-Zitlin, A Richardson
Apr 30, 1998·Physiological Reviews·K B Beckman, B N Ames
Sep 25, 1999·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·S VittoriniE Bergamini
Oct 18, 2000·Free Radical Biology & Medicine·E Cadenas, K J Davies
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·J R RequenaE R Stadtman
Jun 28, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R GredillaG Barja
Aug 23, 2001·Proceedings of the National Academy of Sciences of the United States of America·M L HamiltonA Richardson
Sep 5, 2002·Free Radical Biology & Medicine·Rajindar S SohalWilliam C Orr
Dec 20, 2002·Journal of Nutritional Science and Vitaminology·Byung Pal YuMichihiro Sugano
Jun 17, 2003·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·David R SellVincent M Monnier
Sep 13, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·A J Lambert, B J Merry
Nov 1, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Sharon JudgeChristiaan Leeuwenburgh

❮ Previous
Next ❯

Citations

May 17, 2005·Mechanisms of Ageing and Development·B J Merry
Jun 16, 2005·Mechanisms of Ageing and Development·Maria Cristina RuizReinald Pamplona
Feb 3, 2009·Journal of Toxicology and Environmental Health. Part a·Mohammed AzabKarem Alzoubi
May 9, 2006·Antioxidants & Redox Signaling·Alberto SanzGustavo Barja
Mar 17, 2010·Rejuvenation Research·Katherine OpalachLucia Notterpek
Sep 8, 2010·Cardiovascular Research·Elena M V de CavanaghLeón Ferder
Apr 9, 2005·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Adrian J Lambert, Brian J Merry
Aug 17, 2006·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Sally C FaulksA J Hulbert
Apr 25, 2007·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Victoria AyalaReinald Pamplona
Sep 27, 2007·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Qitao RanArlan Richardson
Jun 1, 2013·Lipids in Health and Disease·Tiina SolakiviSeppo T Nikkari
Oct 26, 2005·Lipids in Health and Disease·T SolakiviS T Nikkari
Oct 17, 2013·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·A J HulbertSarah K Abbott
Jul 11, 2012·International Journal of Cell Biology·Pamela Boon Li Pun, Michael P Murphy
Jun 8, 2013·Free Radical Biology & Medicine·Michael E WalshHolly Van Remmen
Jan 30, 2013·Journal of Lipid Research·Rebecca L SmathersDennis R Petersen
Aug 16, 2011·Molecular Aspects of Medicine·John R Speakman, Sharon E Mitchell
Mar 30, 2010·Revista española de geriatría y gerontología·Alba NaudíReinald Pamplona
Dec 13, 2006·Aging Cell·Teresa G Valencak, Thomas Ruf
Mar 11, 2005·Journal of Theoretical Biology·A J Hulbert
Jan 4, 2012·Mechanisms of Ageing and Development·Sarah HempenstallColin Selman
Mar 5, 2013·Experimental Gerontology·Ines Sanchez-Roman, Gustavo Barja
Dec 20, 2011·Journal of Molecular and Cellular Cardiology·Natasha H BankeE Douglas Lewandowski
Apr 1, 2006·Biochimica Et Biophysica Acta·Reinald Pamplona, Gustavo Barja
Sep 7, 2016·Molecular and Cellular Endocrinology·Lorna MulveyColin Selman
Apr 12, 2013·Experimental Biology and Medicine·Martin J J RonisThomas M Badger
Sep 29, 2006·Anesthesiology·Stanley Muravchick, Richard J Levy
Mar 2, 2018·Cell Death & Disease·Justyna JanikiewiczMariusz R Wieckowski
Mar 7, 2006·Free Radical Research·M SorensenG Barja
Oct 12, 2007·Physiological Reviews·A J HulbertW A Buttemer
May 28, 2005·Endocrinology·Ricardo Gredilla, Gustavo Barja
May 3, 2015·American Journal of Physiology. Heart and Circulatory Physiology·Elena M V de CavanaghLeón Ferder
Sep 10, 2019·Frontiers in Cell and Developmental Biology·Ornella MoltedoGiuseppina Amodio
Sep 26, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mariona JovéReinald Pamplona
Nov 19, 2020·Antioxidants·Mariona JovéReinald Pamplona

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Aging (Keystone)

This feed focuses on cellular aging with emphasis on the mitochondria, autophagy, and metabolic processes associated with aging and longevity. Here is the latest research on cell aging.

Cell Aging

This feed focuses on cellular aging with emphasis on mitochondria, autophagy, and metabolic processes associated with aging and longevity. Here is the latest research on cell aging.