Effect of aging on the pluripotential capacity of human CD105+ mesenchymal stem cells

European Journal of Heart Failure
Santiago RouraAntoni Bayes-Genis

Abstract

Whether aging modifies mesenchymal stem cell (MSC) properties is unknown. To compare the differentiation capacity of human CD105(+) MSCs obtained from young and elderly donors. Cells were obtained from young (n=10, 24+/-6.4 years) and elderly (n=9, 77+/-8.4 years) donors. Cell senescence was assessed by telomere length assays and lipofuscin accumulation. Cell pluripotentiality was analysed by adipogenic and osteogenic induction media, and myocyte phenotype was attempted with 5-azacytidine (5-AZ). Immunofluorescence, Western blot, transmission electron microscopy and fluo-4 confocal imaging were used to analyse the sarcomere, gap junctions and Ca(2+) dynamics. Cells obtained from young and elderly donors showed no significant differences in relative telomere length (40.1+/-6.4% and 40.3+/-3.6%, p=0.9) and lipofuscin accumulation. Adipogenic and osteogenic potential of CD105(+) MSCs was demonstrated. 5-AZ induced increased expression of sarcomeric proteins without complete sarcomere organization. Treated cells also showed increased presence of connexin-43 both in young and old donor-derived cells. Intercellular communications were verified by the observation of gap junctions and passage of Ca(2+) between neighbouring cells. Spont...Continue Reading

References

Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R C AllsoppC B Harley
May 31, 1990·Nature·C B HarleyC W Greider
Feb 1, 1997·Journal of Cellular Biochemistry·N JaiswalS P Bruder
Sep 1, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S A KuznetsovP G Robey
Aug 1, 1998·Nucleic Acids Research·M HultdinG Roos
Apr 2, 1999·Science·M F PittengerD R Marshak
Aug 16, 2001·Proceedings of the National Academy of Sciences of the United States of America·D OrlicP Anversa
Jan 5, 2002·The New England Journal of Medicine·Federico QuainiPiero Anversa
Jan 24, 2002·Nature·Piero Anversa, Bernardo Nadal-Ginard
Sep 21, 2002·Circulation Research·Chunhui XuMelissa K Carpenter
Nov 26, 2002·Cardiovascular Research·Antoni Bayes-GenisJuan Cinca
Feb 28, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jeffrey L SpeesDarwin J Prockop
Dec 3, 2003·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Dominik ParschWiltrud Richter
May 19, 2004·The International Journal of Biochemistry & Cell Biology·Alexei Terman, Ulf T Brunk
Jun 24, 2004·Experimental Cell Research·Ricardo de la FuenteAntonio Bernad
Jul 10, 2004·Circulation Research·Mark F Pittenger, Bradley J Martin

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Citations

Oct 3, 2013·Biogerontology·Hadas Raveh-AmitAndras Dinnyes
Nov 11, 2010·Human Gene Therapy·Yeong-Hoon ChoiChristof Stamm
May 19, 2010·Journal of Periodontology·Karina Gonzales SilvérioFrancisco Humberto Nociti
Dec 6, 2008·Folia Histochemica Et Cytobiologica·Danuta JarochaMarcin Majka
Mar 23, 2011·Clinical Orthopaedics and Related Research·Isaac E EricksonRobert L Mauck
Aug 5, 2009·Stem Cell Reviews and Reports·Marcus JägerRüdiger Krauspe
Apr 5, 2014·Journal of Endodontics·Misako Nakashima, Koichiro Iohara
Jul 8, 2008·Experimental Gerontology·Christof StammRoland Hetzer
Sep 25, 2007·Transplantation Proceedings·C Prat-VidalA Bayes-Genis
Aug 11, 2007·Journal of Biomedical Materials Research. Part a·Caren PetrieEdward Botchwey
Dec 14, 2011·Journal of Tissue Engineering and Regenerative Medicine·Isabel MoscosoNieves Domenech
May 20, 2011·Cell Biology International·Kamila GalaLeszek Pączek
May 3, 2012·Annals of the New York Academy of Sciences·Santiago RouraAntoni Bayes-Genis
Jul 11, 2013·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Melanie VoglMonika Herten
Feb 19, 2015·Journal of Tissue Engineering and Regenerative Medicine·Rodrigo A SomozaCaroline Weinstein-Oppenheimer
Dec 18, 2013·Ageing Research Reviews·Huiqiang ChenJian'an Wang
Jan 29, 2014·Experimental Gerontology·Koichiro IoharaMisako Nakashima
Apr 4, 2015·Journal of Animal Science and Biotechnology·Nuradilla Mohamad-FauziJames D Murray
Aug 18, 2010·Journal of Molecular and Cellular Cardiology·Antoni Bayes-GenisJuan Carlos Izpisúa Belmonte
Jul 14, 2007·Developmental Biology·Maria L ManciniCalvin P H Vary
Nov 27, 2014·Journal of Radiation Research·Mohammad S IslamSusanta K Hui
May 5, 2016·Cellular and Molecular Life Sciences : CMLS·Miaohua MoYaojiong Wu
Jul 6, 2016·Frontiers in Endocrinology·Carolyne FalankMichaela R Reagan

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