Effect of agitation speed on the morphology of Aspergillus niger HFD5A-1 hyphae and its pectinase production in submerged fermentation

World Journal of Biological Chemistry
Darah IbrahimSheh-Hong Lim

Abstract

To investigate the impact of agitation speed on pectinase production and morphological changing of Aspergillus niger (A. niger) HFD5A-1 in submerged fermentation. A. niger HFM5A-1 was isolated from a rotted pomelo. The inoculum preparation was performed by adding 5.0 mL of sterile distilled water containing 0.1% Tween 80 to a sporulated culture. Cultivation was carried out with inoculated 1 × 10(7) spores/mL suspension and incubated at 30 °C with different agitation speed for 6 d. The samples were withdrawn after 6 d cultivation time and were assayed for pectinase activity and fungal growth determination. The culture broth was filtered through filter paper (Whatman No. 1, London) to separate the fungal mycelium. The cell-free culture filtrate containing the crude enzyme was then assayed for pectinase activity. The biomass was dried at 80 °C until constant weight. The fungal cell dry weight was then expressed as g/L. The 6 d old fungal mycelia were harvested from various agitation speed, 0, 50, 100, 150, 200 and 250 rpm. The morphological changing of samples was then viewed under the light microscope and scanning electron microscope. In the present study, agitation speed was found to influence pectinase production in a batch cul...Continue Reading

References

Apr 2, 1998·Journal of Industrial Microbiology & Biotechnology·M C Maldonado, A M Strasser de Saad
Dec 11, 2003·Biotechnology Advances·Maria Papagianni
Jan 18, 2005·Journal of Biotechnology·J L Casas LópezY Chisti
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Citations

Mar 11, 2018·Applied Microbiology and Biotechnology·Asma TimoumiNathalie Gorret
May 17, 2018·Applied Microbiology and Biotechnology·Joseph A BentilAnne S Meyer
Jan 15, 2019·Mycobiology·Piyangkun LueangjaroenkitLerluck Chitradon
Aug 12, 2019·Bioprocess and Biosystems Engineering·F Bajoul KakahiF Delvigne
Jun 30, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Siti Marhaida MustafaHesham Ali El-Enshasy
Mar 28, 2021·Bioresource Technology·Lijian LengHuajun Huang
Apr 30, 2021·Fungal Biology·Radoslav AbrashevMaria Angelova
Jul 27, 2021·Journal of Basic Microbiology·Ekaterina KrumovaMaria Angelova

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