Effect of air drying on bacterial viability: A multiparameter viability assessment

Journal of Microbiological Methods
Andreas NockerFrank Schuren

Abstract

The effect of desiccation on the viability of microorganisms is a question of great interest for a variety of public health questions and industrial applications. Although viability is traditionally assessed by plate counts, cultivation-independent methods are increasingly applied with the aim to gain more insight into why cells might not form colonies and to optimize production processes. To evaluate their usefulness, we applied in this study a multiparameter viability assay to selected bacteria (Escherichia coli, Pseudomonas aeruginosa, Enterococcus hirae, and Staphylococcus aureus) subjected to air-drying in the absence or presence of supplements. Tests included growth on solid culture medium and the measurement of membrane integrity, membrane potential, esterase and respiratory activities using fluorescent dyes. All measured parameters were responsive to desiccation stress. Results suggested that extending plate count analysis with cultivation-independent methods can greatly enhance resolution especially for moderate stress conditions, which do not get reflected in plate counts due to cellular recovery. Whereas plate counts reflect the final effect on viability, immediate measurement of cellular functions provides a snapsho...Continue Reading

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Citations

Feb 21, 2014·Microbial Cell Factories·Rasoul ShafieiPhilippe Thonart
Jan 25, 2016·International Journal of Food Microbiology·Catherine M BurgessOdile Tresse
Dec 17, 2014·Microbiology·Dorota Kuczyńska-WiśnikEwa Laskowska
Jan 3, 2013·Microbiological Research·Vanessa Nessner KavamuraItamar Soares de Melo
Jul 30, 2017·Applied and Environmental Microbiology·Kenneth C MalcolmJerry A Nick
Oct 3, 2018·MicrobiologyOpen·Sabine Zeidler, Volker Müller
Nov 14, 2018·Critical Reviews in Food Science and Nutrition·Sarah L JonesKristen E Gibson
Feb 20, 2019·Environmental Technology·Tangui LéziartAndreas Nocker
Nov 27, 2019·The Journal of Microbiology·Yoonjae ShinWoojun Park
Jul 22, 2018·Applied Microbiology and Biotechnology·Matthew Chekwube Enebe, Olubukola Oluranti Babalola
Mar 23, 2018·Frontiers in Microbiology·Annelein MeisnerWim H van der Putten
Dec 6, 2017·Microbial Biotechnology·Teresa BerningerAngela Sessitsch
Jul 25, 2019·Environmental Science and Pollution Research International·Sivagnanam SilambarasanVelu Rajesh Kannan
Mar 15, 2020·Applied Microbiology and Biotechnology·Vincent Robert Guy Greffe, Jan Michiels
Jan 5, 2021·European Journal of Orthopaedic Surgery & Traumatology : Orthopédie Traumatologie·Juliette CieslinskiFelipe Francisco Tuon
Jan 8, 2021·The ISME Journal·Annelein MeisnerAnders Priemé
Aug 28, 2019·Current Opinion in Microbiology·Ling Xu, Devin Coleman-Derr

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