Effect of alanine ester substitution and other structural features of lipoteichoic acids on their inhibitory activity against autolysins of Staphylococcus aureus.

Journal of Bacteriology
W FischerH U Koch

Abstract

Native substitution with the D-alanine ester of lipoteichoic acids (LTAs) affects their immunological properties, the capacity to bind divalent cations, and LTA carrier activity. In this study we tested the influence of the D-alanine ester on anti-autolytic activity, using extracellular autolysin from Staphylococcus aureus and nine LTAs with alanine/phosphorus molar ratios of between 0.23 and 0.71. The inhibitory activity, highest with alanine-free LTA, exponentially decreased with increasing alanine content, approaching zero at substitutions of greater than 0.6. Correspondingly, dipolar ionic phospholipids were not inhibitory, in contrast to negatively charged ones. Glycosylation of LTA up to an extent of 0.5 did not depress inhibitory activity, and even at a degree of 0.8 the effect was comparatively small. On comparison of LTAs from various sources, differences in lipid structures and chain lengths were without effect. The inhibitory activity drastically decreased when the glycolipid carried a single glycerophosphate residue or the hydrophilic chain had the unusual structure [6 leads to Gal(alpha 1--6)Gal(alpha 1--3)Gro-(2 comes from 1 alpha Gal)-P]n, in which digalactosyl moieties connect the alpha-galactosylated glyceropho...Continue Reading

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Citations

Jan 1, 1987·Critical Reviews in Microbiology·R J Doyle, A L Koch
Jan 15, 1985·European Journal of Biochemistry·T Briese, R Hakenbeck
Jul 1, 1983·European Journal of Biochemistry·W FischerR Haas
Feb 13, 2001·Antimicrobial Agents and Chemotherapy·G Schrader-Fischer, B Berger-Bächi
Feb 13, 2008·FEMS Microbiology Reviews·Waldemar VollmerSimon Foster
Dec 22, 1989·European Journal of Biochemistry·J Schurek, W Fischer
Apr 1, 1996·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·J WeckeW Fischer
Jan 9, 2007·Journal of Bacteriology·Angelika Gründling, Olaf Schneewind
Jul 10, 2007·Journal of Bacteriology·Sang-Joon Ahn, Robert A Burne
Sep 13, 2013·Annual Review of Microbiology·Stephanie BrownSuzanne Walker
Dec 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Francis C Neuhaus, James Baddiley
May 1, 1994·Medical Microbiology and Immunology·W Fischer
May 24, 2011·Microbiology·Nicole E McCormickSong F Lee
Sep 29, 2020·Critical Reviews in Microbiology·Alexander V GrishinVladimir G Lunin
Apr 1, 1996·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·F C NeuhausQ Zhang
Mar 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Kelly C Rice, Kenneth W Bayles
May 8, 2007·Proceedings of the National Academy of Sciences of the United States of America·Angelika Gründling, Olaf Schneewind
Jul 17, 2013·Microbiology·Nathalie T ReichmannAngelika Gründling
Oct 1, 1991·Oral Microbiology and Immunology·E A Thibodeau, C M Ford
Apr 17, 2007·Applied and Environmental Microbiology·Mónica Perea VélezSigrid C J De Keersmaecker
Apr 22, 2017·Proceedings of the National Academy of Sciences of the United States of America·Assaf RazVincent A Fischetti
Mar 15, 2018·Frontiers in Microbiology·Ki Bum AhnSeung Hyun Han
Jul 6, 2019·Frontiers in Microbiology·Soyoung JeongSeung Hyun Han

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