Effect of Albumin in Combination With Mannitol on Whole-blood Coagulation In Vitro Assessed by Thromboelastometry

Journal of Neurosurgical Anesthesiology
Kadri LillemäeTomi T Niemi

Abstract

Albumin and mannitol may interfere with hemostasis, but their coinfluence is unclear. We aimed to determine the effects of albumin alone and in combination with mannitol or Ringer acetate (RAC) on hemostasis in crossover in vitro study. From citrated fresh whole blood withdrawn from 10 volunteers, we prepared 2.5, 5, 10, 15, and 20 vol% dilutions of 4% albumin (Alb group). Each sample was thereafter diluted by 15% mannitol (Alb/Man group) or RAC (Alb/RAC group) at a ratio of 9:1. Using thromboelastometry, FibTEM (fibrinogen ROTEM) and ExTEM (extrinsic ROTEM) tests were performed. A 20 vol%, but not 2.5 to 15 vol% dilution of albumin caused a prolonged clot formation time, α-angle decrease, and maximum clot firmness (MCF) weakening compared with undiluted sample (P<0.05). Clot formation time prolonged more in Alb5/Man than in Alb5 and Alb5/RAC dilution (P<0.05). In Alb2.5/Man, Alb10/Man, and Alb15/Man, dilution α-angle was lower than in corresponding Alb/RAC and Alb-group dilutions (P<0.05). In ExTEM, MCF decreased similarly in every dilution of Alb/Man and Alb/RAC compared with Alb group (P<0.05). In FibTEM, MCF decreased more in Alb10/Man than in Alb10/RAC dilution (P<0.05). In up to 15 vol% dilutions, albumin alone did not im...Continue Reading

References

Oct 1, 1981·Journal of Neurosurgery·A M BurkeC Cerri
Nov 14, 2002·Anaesthesia·K EksethO Engdahl
Mar 9, 2005·Acta Anaesthesiologica Scandinavica·T T Niemi, A H Kuitunen
May 5, 2005·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Thomas LangMeret Lakner
May 22, 2007·Journal of Neurotrauma·Susan L BrattonDavid W Wright
Sep 1, 2007·The New England Journal of Medicine·UNKNOWN SAFE Study InvestigatorsShirley Vallance
Oct 10, 2009·Journal of Neurosurgical Anesthesiology·Ann-Christine LindroosTomi Niemi
May 26, 2011·The Journal of Trauma·Daniel N DarlingtonMichael A Dubick
Sep 29, 2011·Journal of Neurosurgery·Martin M MortazaviWinfield Fisher
Dec 1, 2012·Journal of Neurotrauma·D James CooperUNKNOWN Australian and New Zealand Intensive Care Society Clinical Trials Group
Jan 22, 2013·Clinical Journal of the American Society of Nephrology : CJASN·Andrew D Shaw, John A Kellum
Mar 28, 2013·World Journal of Emergency Surgery : WJES·Ajith SankarankuttySandro Rizoli
Nov 19, 2015·Journal of Neurosurgical Anesthesiology·Joaquín Hernández-PalazónSebastián Burguillos-López

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Citations

Jan 29, 2019·Journal of Neurosurgical Anesthesiology·Jeffrey J Pasternak
Jan 14, 2020·Journal of Neurosurgical Anesthesiology·Heung Kan Ma, John F Bebawy

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Software Mentioned

ExTEM
GraphPad Prism
FibTEM
GraphPad
Statistical Package for Social Sciences

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