Effect of alpha-lactalbumin on aerosol-OT phase structures in oil/water mixtures

The Journal of Physical Chemistry. B
Jun Y Kim, Stephanie R Dungan

Abstract

The ability of water-soluble, globular proteins to tune surfactant/oil/water self-assemblies has potential for the formation of biocompatible microemulsions and also plays a role in protein function at biological interfaces. In this work, we examined the effect of the protein alpha-lactalbumin on Aerosol-OT (AOT) phase structures in equivolume mixtures of oil and 0.1 M brine. In this pseudo-ternary system, surfactants are free to move to either oil or water phase to adopt phase structures close to the spontaneous curvature of the surfactants. Using small-angle X-ray scattering, we observed that addition of this protein changed the spontaneous curvature of the surfactant monolayer substantially. In the absence of protein, AOT adopted a negative spontaneous curvature to form spherical w/o microemulsion droplets. When less than 1 wt % of alpha-lactalbumin was added into the system, the w/o droplets became nonspherical and larger in volume, corresponding to an increase in water uptake into the droplets. As the protein-to-surfactant ratio increased, protein, surfactant, and oil increasingly partitioned toward the aqueous phase. There the protein triggered the formation of o/w microemulsions with a positive spontaneous curvature. The...Continue Reading

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Citations

Aug 31, 2010·The European Physical Journal. E, Soft Matter·S AbelM Marchi
Feb 22, 2011·Physical Chemistry Chemical Physics : PCCP·Jianling ZhangYuanhui Liu
Dec 22, 2020·Chemical Communications : Chem Comm·Feijie LiuTiancheng Mu
Jul 15, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Jun Y Kim, Stephanie R Dungan

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