Effect of aluminium on expression and processing of amyloid precursor protein

Journal of Neuroscience Research
D NeillJ A Edwardson

Abstract

The environmental agent aluminium has been extensively investigated for a potential role in the aetiology of Alzheimer's disease. Despite many investigations there is at present no definite proof for any involvement. If aluminium is involved it is possible that its action is mediated through interaction with the synthesis or processing of amyloid precursor protein (APP). The present study compared aluminium loaded IMR-32 neuroblastoma cells and rat brains with control cells and brains to determine if aluminium affected APP expression and/or processing. In the IMR-32 model system aluminium had no effect on steady-state APP mRNA levels or on the ratio of individual isoforms. It also had no quantitative or qualitative effect on APP-immunoreactive bands detected in protein extracts from conditioned medium of these cells. In total cell extracts, aluminium reduced the intensity of APP-immunoreactive bands between 120-105 kDa but had no effect on a 9 kDa band. In rat brains, aluminium had no effect on APP-immunoreactive bands from soluble or insoluble-membranous extracts. The results, in general, provide no evidence for any effect of aluminium on APP expression or processing.

References

Jul 1, 1992·Nature Genetics·J Hardy
Jul 8, 1991·Neuroscience Letters·J P AndersonN K Robakis
Jan 1, 1991·Biology of Metals·S J McGregorD Halls
Aug 1, 1990·Journal of Neurochemistry·B Shi, A Haug
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M R PalmertS G Younkin
Apr 29, 1989·Lancet·J D Birchall, J S Chappell
Nov 1, 1989·The Canadian Journal of Neurological Sciences. Le Journal Canadien Des Sciences Neurologiques·R W JacobsA B Scheibel
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·I Grundke-IqbalL I Binder
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C L MastersK Beyreuther
May 16, 1984·Biochemical and Biophysical Research Communications·G G Glenner, C W Wong
Nov 1, 1994·Journal of Neuroscience Research·D NeillD Allsop

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Citations

Jan 30, 2010·Cell Biology and Toxicology·Alessandro CastorinaVelia D'Agata
Mar 17, 2012·Environmental Toxicology·Punita BhasinD K Dhawan
May 18, 1999·Molecular and Chemical Neuropathology·S C BondyS Guo-Ross
Mar 10, 2001·Reviews in the Neurosciences·P K Panegyres
Apr 16, 2003·International Journal of Experimental Pathology·M G AbubakarG A A Ferns
Jul 10, 1999·Neuroscience Letters·S C Bondy, A Truong

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