Effect of aluminium on membrane potential and ion fluxes at the apices of wheat roots

Functional Plant Biology : FPB
Tim WherrettSergey Shabala

Abstract

Aluminium (Al) tolerance in wheat (Triticum aestivum L.) is associated with the Al-activated efflux of malate and K+ from the root apices. We tested the hypothesis that these Al-activated ion fluxes would induce changes in the membrane potential (Vm) and that these responses would differ between wheat genotypes that differ in Al tolerance. Within minutes of exposing wheat roots to 50 μm AlCl3, a significant depolarisation was measured in the Al-tolerant ET8 genotype but not in a near-isogenic, Al-sensitive genotype, ES8. We investigated the ion movements that may be responsible for these changes in Vm by measuring real-time fluxes of Cl-, H+ and K+ at the root apices of wheat seedlings using the non-invasive microelectrode ion flux estimation (MIFE) technique. Addition of 50 μm AlCl3 to the bathing solution stimulated an increase in K+ efflux and H+ influx in ET8 but not in ES8. The differences between the genotypes were sustained for 24 h and were observed only at the elongating zone and not the meristematic zone. After 24 h Al increased Cl- influx in ET8 but inhibited ES8 influx in a dose-dependent manner. These results provide new temporal and spatial information on the Al-activated ion fluxes from intact wheat plants.

References

Aug 31, 2000·International Review of Cytology·H Matsumoto
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·P R RyanS D Tyerman
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·PR RyanDL Jones
May 30, 2001·Trends in Plant Science·J F MaE Delhaize
Sep 12, 2002·Plant Physiology·L. A. Papernik, L. V. Kochian
Feb 1, 1995·Plant Physiology·E. Delhaize, P. R. Ryan
Feb 12, 2004·The Plant Journal : for Cell and Molecular Biology·Takayuki SasakiHideaki Matsumoto
Oct 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Emmanuel DelhaizeHideaki Matsumoto
May 1, 2002·Functional Plant Biology : FPB·Sergey Shabala, Andrew Knowles

❮ Previous
Next ❯

Citations

May 26, 2010·Journal of Experimental Botany·Jayakumar BoseZed Rengel
Mar 1, 2006·Plant Signaling & Behavior·Sung-Ju Ahn, Hideaki Matsumoto
Dec 12, 2007·The Plant Journal : for Cell and Molecular Biology·Miguel A PiñerosLeon V Kochian
Dec 23, 2014·Journal of Agricultural and Food Chemistry·Weilan ZhangXingmao Ma

❮ Previous
Next ❯

Methods Mentioned

BETA
enzymatic assay

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.